AB252136

PE Anti-Mesothelin antibody [EPR19025-42]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

1 Images

Flow Cytometry - PE Anti-Mesothelin antibody [EPR19025-42] (AB252136)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-Mesothelin antibody [EPR19025-42] (AB252136)

Overlay histogram showing HeLa cells stained with ab252136 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab252136) (1x 10⁶ in 100μl at 2.5 μg/ml (1/200)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42]
Unconjugated

Anti-Mesothelin antibody [EPR19025-42]
Carrier free

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)
Carrier free

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19025-42

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunology 174:310-321 PubMed39746895

2025

Co-Expression of Dominant-Negative TGF-β Receptor 2 Enhances the Therapeutic Efficacy of Novel TREM1/DAP12-BB-Based CAR-T Cells in Solid Tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Sichao Zhu,Jianping Hu,Jie Lin,Chen Wang,Enxiu Wang

Frontiers in oncology 13:1239957 PubMed38162496

2024

BME-free primary patient-specific organoids obtained with a one-day mimicking method to replicate the corresponding tumor for personalized treatment options.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhu,Zhechun Ding,Yini Wang,Qing Wu,Dongmei Chen,Luanhong Wang,Yuancheng Li,Yao Yao,Jiman Huang,Yun Li,Xiaojing Wang,Yanchun Lin,Tian Guan,Haoyu Zeng,Congzhu Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Mesothelin antibody [EPR19025-42]

Flow Cytometry - PE Anti-Mesothelin antibody [EPR19025-42] (AB252136)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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