Mouse Monoclonal HA11 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein.
Images
![Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25547), expandable thumbnail](https://content.abcam.com/products/images/pe-mhc-class-1-h2-db-h2-d1-antibody-27-11-13-ab25547--flow-cytometry-img170299.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25547)")
Publications
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- Cancer immunology research 10:1028-10382022KDM5A Inhibits Antitumor Immune Responses Through Downregulation of the Antigen-Presentation Pathway in Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Liu,Jianhuang Lin,Wei Zhou,Renyta Moses,Zhongping Dai,Andrew V Kossenkov,Ronny Drapkin,Benjamin G Bitler,Sergey Karakashev,Rugang ZhangPubMed 35726891
- The Journal of experimental medicine 210:1807-212013Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn A H Hoerter et. al.PubMed 23940257
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.2 µg for 106 Cells | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Involved in the presentation of foreign antigens to the immune system.
Alternative names
H-2D(B), H2-D1
Recommended products
Mouse Monoclonal HA11 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein. The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 27-11-13
- Purification technique
- Affinity purification
- Specificity
ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
- Epitope
- This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MHC Class I H2-D1 also known as H2-Db is an important protein within the major histocompatibility complex. This protein carries a molecular mass of approximately 45 kDa. H2-Db is expressed on the surface of almost all nucleated cells. As a protein it plays an important role in immune response by presenting peptide antigens to cytotoxic T cells. Other similar proteins like H2-Kb and H2-Kd are also involved in antigen presentation though each has distinct peptide binding preferences.
- Biological function summary
The MHC Class I H2-Db interacts with peptides derived from cytosolic proteins and loads them onto its structure. It forms a complex with beta-2 microglobulin and presents these peptides on the cell surface. This action is essential in signaling to CD8+ T cells which scan for non-self peptides originating from infections or cellular abnormalities. The method by which H2-Db binds peptides ensures precise immune surveillance.
- Pathways
H2-Db operates prominently in the antigen processing and presentation pathway. This pathway plays an important role in immune response and involves proteases that generate peptide fragments. H2-Db interacts with transporter associated with antigen processing (TAP) proteins to bind peptides enabling effective display of antigens. Furthermore its activity connects with other immune proteins such as H2-Kb in presenting a comprehensive range of antigens to immune cells.
- Associated diseases and disorders
The involvement of H2-Db in autoimmune diseases and viral infections is significant. Certain autoimmune conditions arise when self-antigens are mistakenly presented triggering an immune response. Infections like viral hepatitis show how pathogens can manipulate H2-Db to evade immune detection. Tumor cells may also exploit H2-Db's antigen-presenting function. Proteins such as H2-Kb may interact similarly highlighting the need for MHC Class I diversity in combating disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547), expandable thumbnail](https://content.abcam.com/products/images/pe-mhc-class-1-h2-db-h2-d1-antibody-27-11-13-ab25547--flow-cytometry-img170299.jpg "Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547)")
Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547)
Flow cytometric analysis of mouse BALB/c mouse splenocytes stained for MHC Class 1 H2 Db/H2-D1 using ab25547 at 0.2 μg/106 cells.
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