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AB25547

PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

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(2 Publications)

Mouse Monoclonal HA11 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein.

View Alternative Names

H-2D(B), H2-D1

1 Images
Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25547)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25547)

Flow cytometric analysis of mouse BALB/c mouse splenocytes stained for MHC Class 1 H2 Db/H2-D1 using ab25547 at 0.2 μg/106 cells.

  • Unconjugated

    Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

27-11-13

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.

Specificity

ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.

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Alternative Products

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AB25028

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MHC Class I H2-D1 also known as H2-Db is an important protein within the major histocompatibility complex. This protein carries a molecular mass of approximately 45 kDa. H2-Db is expressed on the surface of almost all nucleated cells. As a protein it plays an important role in immune response by presenting peptide antigens to cytotoxic T cells. Other similar proteins like H2-Kb and H2-Kd are also involved in antigen presentation though each has distinct peptide binding preferences.
Biological function summary

The MHC Class I H2-Db interacts with peptides derived from cytosolic proteins and loads them onto its structure. It forms a complex with beta-2 microglobulin and presents these peptides on the cell surface. This action is essential in signaling to CD8+ T cells which scan for non-self peptides originating from infections or cellular abnormalities. The method by which H2-Db binds peptides ensures precise immune surveillance.

Pathways

H2-Db operates prominently in the antigen processing and presentation pathway. This pathway plays an important role in immune response and involves proteases that generate peptide fragments. H2-Db interacts with transporter associated with antigen processing (TAP) proteins to bind peptides enabling effective display of antigens. Furthermore its activity connects with other immune proteins such as H2-Kb in presenting a comprehensive range of antigens to immune cells.

The involvement of H2-Db in autoimmune diseases and viral infections is significant. Certain autoimmune conditions arise when self-antigens are mistakenly presented triggering an immune response. Infections like viral hepatitis show how pathogens can manipulate H2-Db to evade immune detection. Tumor cells may also exploit H2-Db's antigen-presenting function. Proteins such as H2-Kb may interact similarly highlighting the need for MHC Class I diversity in combating disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the presentation of foreign antigens to the immune system.
See full target information H2-D1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 10:1028-1038 PubMed35726891

2022

KDM5A Inhibits Antitumor Immune Responses Through Downregulation of the Antigen-Presentation Pathway in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Liu,Jianhuang Lin,Wei Zhou,Renyta Moses,Zhongping Dai,Andrew V Kossenkov,Ronny Drapkin,Benjamin G Bitler,Sergey Karakashev,Rugang Zhang

The Journal of experimental medicine 210:1807-21 PubMed23940257

2013

Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC.

Applications

Unspecified application

Species

Unspecified reactive species

John A H Hoerter,Joanna Brzostek,Maxim N Artyomov,Steven M Abel,Javier Casas,Vasily Rybakin,Jeanette Ampudia,Carina Lotz,Janet M Connolly,Arup K Chakraborty,Keith G Gould,Nicholas R J Gascoigne
View all publications
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Product promise

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