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AB95571

PE Anti-MHC class I antibody [34-1-2S]

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(1 Publication)

Mouse Monoclonal HA1W antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing H2-K1 protein.

View Alternative Names

H2-K

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Flow Cytometry - PE Anti-MHC class I antibody [34-1-2S] (AB95571)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-MHC class I antibody [34-1-2S] (AB95571)

Staining of BALB/c splenocytes with 0.125 μg of Mouse IgG2a K Isotype Control PE (blue histogram) or 0.125 μg of ab95571 (purple histogram). Cells in the lymphocyte gate were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

34-1-2S

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing H2-K1 protein. The exact immunogen used to generate this antibody is proprietary information.

P03991

Specificity

ab95571 reacts with the Mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross reacts with K b,s,r,q,p.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Staining the cell sample in a final volume of 100 ÂμL is recommended.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I also known as MHC-1 or simply MHC I is a molecule playing an important role in the immune system. The molecule is a heterodimer composed of a heavy chain weighing around 45 kDa and a light chain. MHC class I molecules are expressed on nearly all nucleated cells in the body. They present peptide fragments from intracellular proteins to CD8+ T cells helping the immune system recognize and destroy infected or cancerous cells. Alternate names like H-2Kd/H-2Dd in mice highlight its role in antigen presentation across different species.
Biological function summary

The molecules of MHC class I form part of the adaptive immune system. They act as a complex with peptides which are derived from proteins degraded by the proteasome. The peptide-MHC I complex travels to the cell surface where it engages with the T cell receptor (TCR) on cytotoxic T cells. This interaction is essential for immune surveillance ensuring the body identifies cells that might compromise health such as those infected by viruses or transformed malignantly.

Pathways

The function of MHC class I integrates into the antigen processing and presentation pathways. It is pivotal in the proteasome-dependent pathway for processing endogenous antigens. Proteins like TAP (transporter associated with antigen processing) assist in transporting peptides into the endoplasmic reticulum for loading onto MHC class I. This pathway is central to cellular immunity and intersects with various immune response cascades.

MHC class I has associations with autoimmune disorders and certain viral infections. Aberrant expression or mutations can lead to diseases like Ankylosing Spondylitis or impact responses to chronic viral infections such as HIV. The involvement of MHC class I in presenting viral peptides often links it to immune evasion mechanisms utilizable by viruses. Hence this protein works closely with immune system proteins like cytokines influencing disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the presentation of foreign antigens to the immune system.
See full target information H2-K1

Additional targets

H2-D1,H2-D1,H2-D1,H2-D1,H2-D1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:372 PubMed40348745

2025

Cellular senescence promotes macrophage-to-myofibroblast transition in chronic ischemic renal disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Xiang-Yang Zhu,Wenqi Ma,Ying Zhang,Fei Yuan,Seo Rin Kim,Hui Tang,Kyra Jordan,Amir Lerman,Tamara Tchkonia,James L Kirkland,Lilach O Lerman
View all publications
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Product promise

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