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PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670), expandable thumbnail
  • Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

1 product for Isotype control

PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG1
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Clone number
R312-MouseIgG1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing no staining in PC-12 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing no staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing no staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Human cervix adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab322670)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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