PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing no staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing no staining in PC-12 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Human cervix adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB322670)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing no staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).