PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
ICC/IF | Flow Cyt (Intra) | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Tested |
Rat | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
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PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing no staining in PC-12 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing no staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/50 (10.0 ug/ml) dilution (Green).
Confocal image showing no staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 488 Anti-beta Tubulin antibody [EPR16774] ab204685 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Human cervix adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Isotype control with ab322670 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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