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AB215670

PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

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(1 Publication)

Mouse Monoclonal MUC1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD227, PUM, MUC1, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

1 Images
Flow Cytometry (Intracellular) - PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB215670)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (AB215670)

Overlay histogram showing A431 cells stained with ab215670 (red line). The cells were fixed with 80% Methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215670, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1 [B11/6] (monoclonal) Phycoerythrin (ab91357) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Carrier free

    Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] - BSA and Azide free

  • Unconjugated

    Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

  • HRP

    HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HMFG1 (aka 1.10.F3)

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" } } }
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Product details

For a more comprehensive guide to this epitope of HMFG1 clone, we recommend the following publications;

Petrakou E et al. 1998: Epitope Mapping of Anti-MUC1 Mucin Protein Core Monoclonal Antibodies. Tumour Biology.PMID: 9422085

Verhoeyen ME et al. 1993: Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody. Immunology. PMID: 7682986

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
See full target information MUC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC veterinary research 21:11 PubMed39789573

2025

Putative effects of moringa oil or its nano-emulsion on the growth, physiological responses, blood health, semen quality, and the sperm antioxidant-related genes in ram.

Applications

Unspecified application

Species

Unspecified reactive species

Rehab F S A Ismail,Wael A Khalil,Sara I Grawish,Karima Gh M Mahmoud,Sameh A Abdelnour,Alaa M A Gad
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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