AB210418

PE Anti-Musashi 1 / Msi1 antibody [EP1302]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Musashi 1 / Msi1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

RNA-binding protein Musashi homolog 1, Musashi-1, MSI1

1 Images

Flow Cytometry (Intracellular) - PE Anti-Musashi 1 / Msi1 antibody [EP1302] (AB210418)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Musashi 1 / Msi1 antibody [EP1302] (AB210418)

Overlay histogram showing Neuro 2A (differentiated) cells stained with ab210418 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210418, 1/2500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302]
660 APC

APC Anti-Musashi 1 / Msi1 antibody [EP1302]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Musashi 1 / Msi1 antibody [EP1302]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Musashi 1 / Msi1 antibody [EP1302]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Musashi 1 / Msi1 antibody [EP1302]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Musashi 1 / Msi1 antibody [EP1302]
Unconjugated

Anti-Musashi 1 / Msi1 antibody [EP1302]
Carrier free

Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1302

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" }, "Chicken": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Quail": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.

Biological function summary

In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.

Pathways

The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.

Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

See full target information MSI1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 26:203-212 PubMed38948130

2024

Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Shunxin Jin,Dapeng Cheng,Hao Chen,Yuxiang Wang,Chao Ji,Zhenzhen Yan,Xiao Fang,Shichu Xiao,Xinling Bi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Musashi 1 / Msi1 antibody [EP1302]

Flow Cytometry (Intracellular) - PE Anti-Musashi 1 / Msi1 antibody [EP1302] (AB210418)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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