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AB72468

PE Anti-Myc tag antibody [9E10]

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(2 Publications)

Anti-Myc tag antibody [9E10] - PE conjugated (ab72468) is a mouse monoclonal antibody detecting Myc tag in Flow Cytometry (Intra).

- Trusted since 2008
1 Images
Flow Cytometry (Intracellular) - PE Anti-Myc tag antibody [9E10] (AB72468)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Myc tag antibody [9E10] (AB72468)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab72468 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Phycoerythrin (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Phycoerythrin conjugated Myc-tag antibody (ab72468) and Alexa Fluor® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myc tag antibody [9E10]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Myc tag antibody [9E10]

  • 660 APC

    APC Anti-Myc tag antibody [9E10]

  • HRP

    HRP Anti-Myc tag antibody [9E10]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Myc tag antibody [9E10]

  • Biotin

    Biotin Anti-Myc tag antibody [9E10] - C-terminal

  • 519 FITC

    FITC Anti-Myc tag antibody [9E10]

  • Biotin

    Biotin Anti-Myc tag antibody [9E10]

  • 657 SureLight® APC

    SureLight® APC Anti-Myc tag antibody [9E10]

  • 576 DyLight® 550

    DyLight® 550 Anti-Myc tag antibody [9E10]

  • 673 DyLight® 650

    DyLight® 650 Anti-Myc tag antibody [9E10]

  • 518 DyLight® 488

    DyLight® 488 Anti-Myc tag antibody [9E10]

  • Unconjugated

    Anti-Myc tag antibody [9E10]

  • Carrier free

    Anti-Myc tag antibody [9E10] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9E10

Isotype

IgG1

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB206486

Anti-Myc tag antibody [9E10]

Recombinant

Western blot - Anti-Myc tag antibody [9E10] (AB206486)

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Complementary Products

Primary Antibodies

AB305269

Alexa Fluor® 488 Anti-HA tag antibody [EPR22819-101]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-HA tag antibody [EPR22819-101] (AB305269)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB237337

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p><p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

What is this antibody validated in?
PE Anti-Myc tag antibody [9E10] (ab72468) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt). Tagged protein detection
Myc tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone 9E10 is also available pre-conjugated to a variety of labels for your convenience – Anti-Myc tag PE [9E10] (ab72468).

Other related products
We have a range of other formats of antibody clone [9E10] also available for your convenience: ab185655, Carrier free - ab236021, PE - ab237249, Alexa Fluor® 488 - ab300546, Alexa Fluor® 647 - ab300731, APC - ab310838, Alexa Fluor® 594 - ab311655, Alexa Fluor® 568 - ab312928, Alexa Fluor® 555 - ab313141, HRP - ab62928, PE - ab72468, SureLight® APC - ab72580, Biotin - ab81658, DyLight® 650 - ab117487, Carrier free - ab117499, FITC - ab117599
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Myc tag
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108287 PubMed38034357

2023

CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arsenios Vlassis,Tanja L Jensen,Marina Mohr,Dominika J Jedrzejczyk,Xiangyou Meng,Gergo Kovacs,Martí Morera-Gómez,Andrea Barghetti,Sergi Muyo Abad,Roland F Baumgartner,Kedar N Natarajan,Lars K Nielsen,Tanya Warnecke,Ryan T Gill

Oncoimmunology 7:e1377874 PubMed29296540

2017

ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy.

Applications

WB

Species

Human

Seandean Lykke Harwood,Ana Alvarez-Cienfuegos,Natalia Nuñez-Prado,Marta Compte,Sara Hernández-Pérez,Nekane Merino,Jaume Bonet,Rocio Navarro,Paul M P Van Bergen En Henegouwen,Simon Lykkemark,Kasper Mikkelsen,Kasper Mølgaard,Frederic Jabs,Laura Sanz,Francisco J Blanco,Pedro Roda-Navarro,Luis Alvarez-Vallina
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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