AB11730

PE Anti-Myeloperoxidase antibody [2C7]

Name: Anti-Myeloperoxidase PE antibody [2C7] (ab11730) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB11730
Price: 545 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal Myeloperoxidase antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MPO.

View Alternative Names

Myeloperoxidase, MPO

Unconjugated

Anti-Myeloperoxidase antibody [2C7]
519 FITC

FITC Anti-Myeloperoxidase antibody [2C7]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2C7

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MPO.

P05164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10 μl of undiluted antibody to label 106 cells in 100 μl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation." } } }

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Product details

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 5% Sucrose, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 14:594170 PubMed33250718

2020

Development of Neuroregenerative Gene Therapy to Reverse Glial Scar Tissue Back to Neuron-Enriched Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Zhuofan Lei,Ziyuan Guo,Zifei Pei,Yuchen Chen,Fengyu Zhang,Alice Cai,Gabriel Mok,Grace Lee,Vishal Swaminathan,Fan Wang,Yuting Bai,Gong Chen

Frontiers in immunology 10:963 PubMed31114589

2019

High-Content Screening Identifies Vanilloids as a Novel Class of Inhibitors of NET Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Elvira Sondo,Roberta Bertelli,Emanuela Pesce,Gian Marco Ghiggeri,Nicoletta Pedemonte

International journal of nephrology 2012:715049 PubMed22792461

2012

Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis.

Applications

Unspecified application

Species

Unspecified reactive species

Susann Patschan,Daniel Patschan,Elvira Henze,Sabine Blaschke,Johannes T Wessels,Gerhard Anton Müller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-Myeloperoxidase antibody [2C7]

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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