AB93525

PE Anti-N Cadherin antibody [8C11]

Name: Anti-N Cadherin PE antibody [8C11] (ab93525) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB93525

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(2 Publications)

Mouse Monoclonal N Cadherin antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Fusion protein corresponding to aa 50-600 of CDH2, fused to CDH2.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

1 Images

Flow Cytometry - PE Anti-N Cadherin antibody [8C11] (AB93525)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-N Cadherin antibody [8C11] (AB93525)

Staining of HeLa cells with PE Mouse IgG1 kappa Isotype Control (blue histogram), or ab93525. Total viable cells were used for analysis.

Unconjugated

Anti-N Cadherin antibody [8C11]
Carrier free

Anti-N Cadherin antibody [8C11] - BSA and Azide free
Biotin

Biotin Anti-N Cadherin antibody [8C11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8C11

Isotype

IgG1

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Fusion protein corresponding to aa 50-600 of CDH2, fused to CDH2. The exact immunogen used to generate this antibody is proprietary information.

P19022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

See full target information CDH2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 278:17269-76 PubMed12604612

2003

N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.

Applications

Species

Unspecified reactive species

James K Wahl,Young J Kim,Janet M Cullen,Keith R Johnson,Margaret J Wheelock

Journal of cell science 114:1567-77 PubMed11282032

2001

N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.

Applications

Flow Cyt

Species

Unspecified reactive species

S Puch,S Armeanu,C Kibler,K R Johnson,C A Müller,M J Wheelock,G Klein

View all publications

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Product promise

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PE Anti-N Cadherin antibody [8C11]

Flow Cytometry - PE Anti-N Cadherin antibody [8C11] (AB93525)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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