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AB18277

PE Anti-NCAM1 antibody [MEM-188]

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(4 Publications)

Mouse Monoclonal NCAM1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human, Mouse, Monkey samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

2 Images
Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277)

Separation of human CD56 positive CD3 negative NK cells (red-filled) from CD56 negative CD3 positive T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab18277 (20 µl reagent / 100 µl of peripheral whole blood).

Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277)

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and ab18277 (20 µl reagent / 100 µl of peripheral whole blood).

  • Unconjugated

    Anti-NCAM1 antibody [MEM-188]

  • 660 APC

    APC Anti-NCAM1 antibody [MEM-188]

  • 519 FITC

    FITC Anti-NCAM1 antibody [MEM-188]

  • 778 PE/Cy7®

    PE/Cy7® Anti-NCAM1 antibody [MEM-188]

  • 675 PerCP

    PerCP Anti-NCAM1 antibody [MEM-188]

  • Biotin

    Biotin Anti-NCAM1 antibody [MEM-188]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-188

Isotype

IgG2a

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse, Human, Monkey

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P13591

Specificity

Human NCAM. Reacts with the 180 kDa isoform characteristic for leukocytes. Reactivity with other NCAM isoforms has not been tested.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood or 10^6 cells in a suspension. <a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood or 10^6 cells in a suspension. <a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood or 10^6 cells in a suspension. <a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

Properties and storage information

Form
Liquid
Purification notes
Purified by size exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: BSA, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 120:e2205371120 PubMed36595695

2023

Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Junil Kim,Michaela Mrugala Rothová,Esha Madan,Siyeon Rhee,Guangzheng Weng,António M Palma,Linbu Liao,Eyal David,Ido Amit,Morteza Chalabi Hajkarim,Vignesh Vudatha,Andrés Gutiérrez-García,Eduardo Moreno,Robert Winn,Jose Trevino,Paul B Fisher,Joshua M Brickman,Rajan Gogna,Kyoung Jae Won

NPJ breast cancer 8:78 PubMed35780131

2022

The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sepideh Soukhtehzari,Richard B Berish,Ladan Fazli,Peter H Watson,Karla C Williams

Nucleic acids research 46:7686-7700 PubMed29931089

2018

The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Pierre Lambert,Sophie Terrone,Guillaume Giraud,Clara Benoit-Pilven,David Cluet,Valérie Combaret,Franck Mortreux,Didier Auboeuf,Cyril F Bourgeois

PloS one 9:e99819 PubMed24932758

2014

Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies.

Applications

Flow Cyt

Species

Mouse

Marco Skardelly,Eileen Hempel,Johannes Hirrlinger,Florian Wegner,Jürgen Meixensberger,Javorina Milosevic
View all publications

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