Mouse Monoclonal NCAM1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human, Mouse, Monkey samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.
Images
![Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277), expandable thumbnail](https://content.abcam.com/products/images/pe-ncam1-antibody-mem-188-ab18277--flow-cytometry-img435990.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277)")
![Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277), expandable thumbnail](https://content.abcam.com/products/images/pe-ncam1-antibody-mem-188-ab18277--flow-cytometry-img435989.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (AB18277)")
Publications
Filter by
- Proceedings of the National Academy of Sciences of the United States of America 120:e22053711202023Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunil Kim,Michaela Mrugala Rothová,Esha Madan,Siyeon Rhee,Guangzheng Weng,António M Palma,Linbu Liao,Eyal David,Ido Amit,Morteza Chalabi Hajkarim,Vignesh Vudatha,Andrés Gutiérrez-García,Eduardo Moreno,Robert Winn,Jose Trevino,Paul B Fisher,Joshua M Brickman,Rajan Gogna,Kyoung Jae WonPubMed 36595695
- NPJ breast cancer 8:782022The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSepideh Soukhtehzari,Richard B Berish,Ladan Fazli,Peter H Watson,Karla C WilliamsPubMed 35780131
- Nucleic acids research 46:7686-77002018The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Pierre Lambert,Sophie Terrone,Guillaume Giraud,Clara Benoit-Pilven,David Cluet,Valérie Combaret,Franck Mortreux,Didier Auboeuf,Cyril F BourgeoisPubMed 29931089
- PloS one 9:e998192014Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies.Applications:Flow CytReactive species:MouseMarco Skardelly,Eileen Hempel,Johannes Hirrlinger,Florian Wegner,Jürgen Meixensberger,Javorina MilosevicPubMed 24932758
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: BSA, PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
Consider this alternative
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Mouse | Expected |
| Monkey | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood or 10^6 cells in a suspension. ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood or 10^6 cells in a suspension. ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Monkey | Dilution info - | Notes Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood or 10^6 cells in a suspension. ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
5 products for Alternative Version
8 products for Alternative Product
Target data
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Alternative names
CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1
Recommended products
Mouse Monoclonal NCAM1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human, Mouse, Monkey samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing NCAM1 protein.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: BSA, PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13591
- Clone number
- MEM-188
- Specificity
Human NCAM. Reacts with the 180 kDa isoform characteristic for leukocytes. Reactivity with other NCAM isoforms has not been tested.
- Concentration
- Purification notes
Purified by size exclusion chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
- Biological function summary
NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.
- Pathways
The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.
- Associated diseases and disorders
NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (ab18277), expandable thumbnail](https://content.abcam.com/products/images/pe-ncam1-antibody-mem-188-ab18277--flow-cytometry-img435990.jpg "Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (ab18277)")
Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (ab18277)
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and ab18277 (20 µl reagent / 100 µl of peripheral whole blood).
![Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (ab18277), expandable thumbnail](https://content.abcam.com/products/images/pe-ncam1-antibody-mem-188-ab18277--flow-cytometry-img435989.jpg "Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (ab18277)")
Flow Cytometry - PE Anti-NCAM1 antibody [MEM-188] (ab18277)
Separation of human CD56 positive CD3 negative NK cells (red-filled) from CD56 negative CD3 positive T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab18277 (20 µl reagent / 100 µl of peripheral whole blood).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com