Skip to main content

Rabbit Recombinant Monoclonal NF-kB p65 phospho S536 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RELA phospho S536.

Be the first to review this product! Submit a review

Images

Flow Cytometry - PE Anti-NF-kB p65 (phospho S536) antibody [NFKBp65S536-B7] (AB278630), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human RELA phospho S536. The exact immunogen used to generate this antibody is proprietary information. Database link Q04206

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

5 µL for 106 Cells

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal NF-kB p65 phospho S536 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human RELA phospho S536.

Alternative names

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human RELA phospho S536. The exact immunogen used to generate this antibody is proprietary information. Database link Q04206
Clone number

NFKBp65S536-B7

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - PE Anti-NF-kB p65 (phospho S536) antibody [NFKBp65S536-B7] (ab278630), expandable thumbnail

    Flow Cytometry - PE Anti-NF-kB p65 (phospho S536) antibody [NFKBp65S536-B7] (ab278630)

    Flow cytometric analysis of HeLa cells untreated and unstained as negative control (blue), untreated (red) or treated with TNFa plus CalA (green) and stained using ab278630.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com