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AB275621

PE Anti-Nicastrin antibody [002]

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(1 Publication)

Rabbit Recombinant Monoclonal Nicastrin antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCSTN aa 1-700.

View Alternative Names

KIAA0253, UNQ1874/PRO4317, NCSTN, Nicastrin

1 Images
Flow Cytometry - PE Anti-Nicastrin antibody [002] (AB275621)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-Nicastrin antibody [002] (AB275621)

Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Nicastrin with ab275621 (Black) compared with an isotype control (Grey).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

002

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human NCSTN aa 1-700. The exact immunogen used to generate this antibody is proprietary information.

Q92542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>10 μl/Test</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nicastrin also known as NAS is an integral component of the gamma-secretase complex. This protein interacts mechanically by assisting in substrate recognition and stabilization within the complex. Nicastrin has a molecular weight of approximately 75 kDa. It is predominantly expressed in various tissues including the brain where it plays significant role in neuronal functions.
Biological function summary

Nicastrin functions as a part of the gamma-secretase complex which also includes presenilin anterior pharynx defective 1 (APH-1) and presenilin enhancer 2 (PEN-2). This complex is essential for intramembrane proteolysis of type I membrane proteins such as amyloid precursor protein (APP) and Notch. The activity of nicastrin influences processes like synaptic organization and neuronal development.

Pathways

Gamma-secretase activity facilitated by nicastrin is central in the Notch signaling pathway and the amyloidogenic pathway. In the Notch pathway it helps release the Notch intracellular domain important for T-cell development. In the amyloidogenic pathway the complex processes APP contributing to amyloid-beta peptide formation. Beta-secretase (BACE1) also plays an important role in this pathway acting upstream of gamma-secretase.

The imbalance in nicastrin function connects it to Alzheimer's disease and certain cancers. Its role in amyloid-beta peptide production links nicastrin to Alzheimer's disease pathogenesis. In cancer the deregulation of Notch signaling in which nicastrin is involved influences tumor progression and survival. The interaction of nicastrin with presenilin is particularly notable in these conditions further emphasizing its significance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10993067, PubMed : 12679784, PubMed : 25043039, PubMed : 26280335, PubMed : 30598546, PubMed : 30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
See full target information NCSTN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular genetics & genomic medicine 12:e70030 PubMed39600113

2024

Pathogenicity of the LDLR c.97C>T (p.Gln33Ter) Mutation in Familial Hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kaihan Wang,Tingting Hu,Mengmeng Tai,Yan Shen,Shaoyi Lin,Yongjuan Guo,Xiaomin Chen
View all publications
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Product promise

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