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AB315144

PE Anti-NKR-P1C antibody [EPR22990-12]

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Rabbit Recombinant Monoclonal NKR-P1C antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD161c, Ly55c, Nkrp1c, Klrb1c, Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, Ly-55c, NKR-P1 40

2 Images
Flow Cytometry - PE Anti-NKR-P1C antibody [EPR22990-12] (AB315144)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-NKR-P1C antibody [EPR22990-12] (AB315144)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab315144 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315144 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cytometry - PE Anti-NKR-P1C antibody [EPR22990-12] (AB315144)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-NKR-P1C antibody [EPR22990-12] (AB315144)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab315144 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315144 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD49b.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22990-12

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKR-P1C also known as NK1.1 is a C-type lectin-like receptor expressed on the surface of natural killer (NK) cells and some T cells. In mice this protein has a molecular mass of approximately 30 to 46 kDa. Researchers often focus on its distribution in cellular compartments which includes lymphoid tissues such as the spleen and thymus. This receptor is known for playing a role in the regulation of immune responses by enabling NK cells to recognize and eliminate target cells.
Biological function summary

NKR-P1C is critical in the activation and inhibition of natural killer cell functions. This protein does not operate alone but often functions as part of an immune receptor complex. It contributes to the balance between activating and inhibitory signals that determine NK cell responses. NKR-P1C can bind to ligands that influence the cytotoxic activity of these cells toward infected or malignant cells.

Pathways

NKR-P1C integrates into important immune signaling pathways such as the NK cell-mediated cytotoxicity pathway. This pathway involves interactions with other receptors like Ly49 and NKG2D which modulate NK cell activities. NKR-P1C plays an essential role in transmitting signals that lead to the release of cytokines and the destruction of afflicted cells coordinating with these receptors to execute immune functions.

NKR-P1C has associations with cancer and viral infections where it influences NK cell interventions against pathologies. During tumor development or viral infection NKR-P1C may change expression patterns potentially affecting immune surveillance and responses. It works in tandem with proteins like perforin and granzymes which are involved in inducing cell death in target cells making it a significant player in immune responses to malignancies and persistent viruses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.
See full target information Klrb1c
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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