AB213198

PE Anti-Oct4 antibody [EPR17980]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Oct4 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3

1 Images

Flow Cytometry (Intracellular) - PE Anti-Oct4 antibody [EPR17980] (AB213198)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Oct4 antibody [EPR17980] (AB213198)

Overlay histogram showing F9 cells stained with ab213198 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213198, 1/1000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Oct4 antibody [EPR17980]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Oct4 antibody [EPR17980]
Unconjugated

Anti-Oct4 antibody [EPR17980]
Carrier free

Anti-Oct4 antibody [EPR17980] - BSA and Azide free (Capture)
Carrier free

Anti-Oct4 antibody [EPR17980] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17980

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.

Biological function summary

Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.

Pathways

Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.

Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene POU5F1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and forms a trimeric complex with SOX2 or SOX15 on DNA. It controls the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206. This protein is critical for early embryogenesis and for maintaining embryonic stem cell pluripotency. This supplementary information is collated from multiple sources and compiled automatically.

See full target information POU5F1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:4713-4732.e19 PubMed38968937

2024

Progestogen-driven B7-H4 contributes to onco-fetal immune tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Yu,Yijian Yan,Shasha Li,Ying Xu,Abhijit Parolia,Syed Rizvi,Weichao Wang,Yiwen Zhai,Rongxin Xiao,Xiong Li,Peng Liao,Jiajia Zhou,Karolina Okla,Heng Lin,Xun Lin,Sara Grove,Shuang Wei,Linda Vatan,Jiantao Hu,Justyna Szumilo,Jan Kotarski,Zachary T Freeman,Stephanie Skala,Max Wicha,Kathleen R Cho,Arul M Chinnaiyan,Samantha Schon,Fei Wen,Ilona Kryczek,Shaomeng Wang,Lieping Chen,Weiping Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Oct4 antibody [EPR17980]

Flow Cytometry (Intracellular) - PE Anti-Oct4 antibody [EPR17980] (AB213198)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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