AB95656

PE Anti-OX40L/TNFSF4 antibody [RM134L]

Name: PE Anti-OX40L/TNFSF4 antibody [RM134L] (ab95656) | Abcam
Brand: Abcam Limited
SKU: AB95656

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(4 Publications)

Rat Monoclonal OX40L/TNFSF4 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Tnfsf4.

View Alternative Names

CD252, Ox40l, Txgp1l, Tnfsf4, Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L

1 Images

Flow Cytometry - PE Anti-OX40L/TNFSF4 antibody [RM134L] (AB95656)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-OX40L/TNFSF4 antibody [RM134L] (AB95656)

Flow cytometry staining of 3-day unstimulated (left) and 3-day Anti-Mouse IgM and Anti-Mouse CD40 stimulated (right) BALB/c splenocytes with 0.25 μg of Rat IgG2b Isotype Control PE (open histogram) or 0.25 μg of ab95656 (filled histogram). Total viable cells were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM134L

Isotype

IgG2b

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Mouse Tnfsf4. The exact immunogen used to generate this antibody is proprietary information.

P43488

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25-0.5 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>Staining the cell sample in a final volume of 100 μl is recommended.</p>" } } }

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Product details

The RM134L monoclonal antibody inhibits the costimulatory activity of Mouse CD252 transfected cells for anti-CD3-stimulated T cell proliferation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OX40L also known as TNFSF4 is a member of the tumor necrosis factor (TNF) ligand family. It is a type II transmembrane protein with a molecular weight of about 32 kDa. OX40L is expressed mainly on activated antigen-presenting cells such as B cells dendritic cells and macrophages. The protein interacts specifically with its receptor OX40 (CD134) which is found on activated T cells mainly CD4+ T cells. These interactions are important for the regulation of immune responses.

Biological function summary

OX40L acts as a costimulatory signal for T cells. It does not work alone; it functions as part of the immune complex involved in enhancing T cell proliferation cytokine production and survival. OX40L enhances the immune response making it a critical modulator of immunity. This protein interaction is essential for promoting an effective adaptive immune response influencing T cell memory and maintaining immune system balance.

Pathways

OX40L is involved in the TNF receptor pathway which plays an important role in cell survival and inflammation. It influences the NF-κB signaling pathway which regulates immune response and cell proliferation. OX40L interactions also involve the Akt pathway important for T cell survival and function. The related proteins in these pathways include notably the OX40 protein and CD317 which participate in immune signaling and regulation.

OX40L is implicated in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The interaction between OX40L and OX40 increases T cell activation contributing to inflammation and disease progression. OX40L's excessive activity has links to these pathologies making it a potential therapeutic target. Another related protein within disease contexts is CD317 connected to immune modulation and control of viral infections and offering further opportunities for targeted treatment strategies involving OX40L.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

See full target information Tnfsf4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1410564 PubMed39007148

2024

Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Rakitina,Alexey I Kuzmich,Olga A Bezborodova,Sofia A Kondratieva,Victor V Pleshkan,Marina V Zinovyeva,Dmitry A Didych,Aleksandr V Sass,Eugene V Snezhkov,Maria B Kostina,Maksim O Koksharov,Irina V Alekseenko

Blood 95:2434-9 PubMed10733518

2000

Blockade of CD134 (OX40)-CD134L interaction ameliorates lethal acute graft-versus-host disease in a murine model of allogeneic bone marrow transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

N Tsukada,H Akiba,T Kobata,Y Aizawa,H Yagita,K Okumura

The Journal of experimental medicine 191:375-80 PubMed10637281

2000

Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis.

Applications

Unspecified application

Species

Unspecified reactive species

H Akiba,Y Miyahira,M Atsuta,K Takeda,C Nohara,T Futagawa,H Matsuda,T Aoki,H Yagita,K Okumura

Journal of immunology (Baltimore, Md. : 1950) 162:7058-66 PubMed10358148

1999

CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells.

Applications

Unspecified application

Species

Unspecified reactive species

H Akiba,H Oshima,K Takeda,M Atsuta,H Nakano,A Nakajima,C Nohara,H Yagita,K Okumura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-OX40L/TNFSF4 antibody [RM134L]

Flow Cytometry - PE Anti-OX40L/TNFSF4 antibody [RM134L] (AB95656)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise