AB93590

PE Anti-P Glycoprotein antibody [UIC2]

Name: PE Anti-P Glycoprotein antibody [UIC2] (ab93590) | Abcam
Brand: Abcam Limited
SKU: AB93590
Price: 275 USD
Availability: InStock

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(11 Publications)

Mouse Monoclonal P Glycoprotein antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Cell preparation containing ABCB1 protein.

View Alternative Names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

1 Images

Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (AB93590)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (AB93590)

Staining of the human 8226/S (blue histogram) and human 8226 DOX40 (purple histogram) cell lines with PE Mouse IgG2a, K Isotype Control (left) or ab93590 (right). Total viable cells were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UIC2

Isotype

IgG2a

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ABCB1 protein. The exact immunogen used to generate this antibody is proprietary information.

P08183

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 5 μl/test for 105-8 cells in 100μl.</p><p><a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

ab93590 has been reported to inhibit MDR1-mediated efflux.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P-glycoprotein also known as P-gp MDR1 transporter or ABCB1 is a well-characterized efflux transporter with a molecular mass of approximately 170 kDa. This protein is expressed in various normal tissues such as the liver intestine kidney blood-brain barrier and placenta. It is located on the cell membrane where it functions to expel a wide variety of substrates out of cells using energy derived from ATP hydrolysis. This capability of pumping out toxins and xenobiotics makes P-glycoprotein an important player in cellular detoxification.

Biological function summary

P-glycoprotein acts as a protective mechanism against toxic substances and drugs within the body. It is a member of the ATP-binding cassette (ABC) transporter family which is critical for their ability to transport molecules across membranes. P-glycoprotein is not typically part of a larger protein complex but can interact with other membrane proteins to facilitate its function. Its role in drug metabolism and excretion is vital for maintaining the balance and elimination of harmful substances.

Pathways

P-glycoprotein plays a significant role in the pharmacokinetics of drugs within the body. This transporter is involved in the multi-drug resistance (MDR) pathway which impacts the efficacy of chemotherapeutic agents. P-glycoprotein also interacts with other proteins like CYP3A4 which is involved in the metabolism of many drugs. These interactions affect the concentration and distribution of therapeutic agents contributing to the body's overall drug response.

P-glycoprotein is closely associated with multi-drug resistance in cancer where its overexpression can lead to reduced effectiveness of chemotherapy. It is also implicated in disorders of the central nervous system like epilepsy where it affects drug delivery to the brain. The protein has connections with CYP3A4 in these contexts where both together influence drug metabolism and efficacy complicating treatment regimens and therapeutic outcomes in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translocates drugs and phospholipids across the membrane (PubMed : 2897240, PubMed : 35970996, PubMed : 8898203, PubMed : 9038218, PubMed : 35507548). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed : 8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed : 2897240, PubMed : 35970996, PubMed : 9038218).

See full target information ABCB1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 11:e2201178 PubMed35668035

2022

Engineering CpG-ASO-Pt-Loaded Macrophages (CAP@M) for Synergistic Chemo-/Gene-/Immuno-Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Wang,Lingpu Zhang,Yan Liu,Linlin Tang,Juan He,Xiaqing Sun,Muhsin H Younis,Daxiang Cui,Haihua Xiao,Dong Gao,Xiang-Yang Kong,Weibo Cai,Jie Song

Analytical and bioanalytical chemistry 413:2021-2031 PubMed33528601

2021

Surface plasmon resonance biosensor combined with lentiviral particle stabilization strategy for rapid and specific screening of P-Glycoprotein ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhong Cao,Yan Cao,Yiwei Shi,Ying Cai,Langdong Chen,Dongyao Wang,Yue Liu,Xiaofei Chen,Zhenyu Zhu,Zhanying Hong,Yifeng Chai

Molecular medicine reports 20:3883-3892 PubMed31485616

2019

Hydroxychloroquine reverses the drug resistance of leukemic K562/ADM cells by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Wang,Zhewen Zhang,Wing Ting Leung,Jing Chen,Juan Yi,Chunmei Ying,Minmin Yuan,Mingyan Wang,Na Zhang,Xuemin Qiu,Ling Wang,Hulai Wei

Blood cancer journal 9:51 PubMed31113932

2019

ABCB1 SNP predicts outcome in patients with acute myeloid leukemia treated with Gemtuzumab ozogamicin: a report from Children's Oncology Group AAML0531 Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Roya Rafiee,Lata Chauhan,Todd A Alonzo,Yi-Cheng Wang,Ahlam Elmasry,Michael R Loken,Jessica Pollard,Richard Aplenc,Susana Raimondi,Betsy A Hirsch,Irwin D Bernstein,Alan S Gamis,Soheil Meshinchi,Jatinder K Lamba

Nature communications 10:1981 PubMed31040273

2019

Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Hui Guo,Chen-Lin Feng,Wen-Xuan Zhang,Zhi-Gang Luo,Hong-Juan Zhang,Ting-Ting Zhang,Chen Ma,Yun Zhan,Rui Li,Song Wu,Zeper Abliz,Cong Li,Xiao-Lin Li,Xiao-Lei Ma,Lu-Lu Wang,Wen-Sheng Zheng,Yan-Xing Han,Jian-Dong Jiang

Drug delivery 26:443-458 PubMed30929529

2019

Surface-engineered polyethyleneimine-modified liposomes as novel carrier of siRNA and chemotherapeutics for combination treatment of drug-resistant cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Livia P Mendes,Can Sarisozen,Ed Luther,Jiayi Pan,Vladimir P Torchilin

Molecules (Basel, Switzerland) 23: PubMed29534440

2018

Chiral Thioxanthones as Modulators of P-glycoprotein: Synthesis and Enantioselectivity Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Lopes,Eva Martins,Renata Silva,Madalena M M Pinto,Fernando Remião,Emília Sousa,Carla Fernandes

Oncotarget 8:66033-66050 PubMed29029490

2017

7-ketocholesterol and 27-hydroxycholesterol decreased doxorubicin sensitivity in breast cancer cells: estrogenic activity and mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Wei Wang,Chiung-Chiao Huang,Pei-Hsin Chou,Yu-Ping Chang,Shouzuo Wei,Frederick Peter Guengerich,Yueh-Ching Chou,Sheng-Fan Wang,Ping-Shan Lai,Pavel Souček,Yune-Fang Ueng

Biochemical pharmacology 85:1257-68 PubMed23415901

2013

Parguerenes: Marine red alga bromoditerpenes as inhibitors of P-glycoprotein (ABCB1) in multidrug resistant human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Cong Huang,Yue-Li Sun,Angela A Salim,Zhe-Sheng Chen,Robert J Capon

Blood 80:2735-9 PubMed1360267

1992

Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

P M Chaudhary,E B Mechetner,I B Roninson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE Anti-P Glycoprotein antibody [UIC2]

Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (AB93590)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise