Mouse Monoclonal Cytokeratin 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.
Images
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-intracellular-img86896.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)")
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-intracellular-img446375.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)")
![Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-img446374.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)")
Publications
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- Cancers 14:2022Modeling Colorectal Cancer Progression Reveals Niche-Dependent Clonal Selection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuria Vaquero-Siguero,Nikolai Schleussner,Julia Volk,Manuel Mastel,Jasmin Meier,Rene JackstadtPubMed 36077793
- Bio-protocol 10:e38222020Magnet-assisted Flow Cytometry of Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPreethi Korangath,Robert IvkovPubMed 33659474
- Theranostics 10:11026-110482020ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKok Suen Cheng et. al.PubMed 33042268
- Nature communications 10:54952019Senescent cell turnover slows with age providing an explanation for the Gompertz law.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmer Karin et. al.PubMed 31792199
- EBioMedicine 46:133-1492019CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukun Sun et. al.PubMed 31375425
- Cancer research 79:2962-29772019Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManjulata Singh et. al.PubMed 30952634
- Journal of immunology (Baltimore, Md. : 1950) 201:1500-15092018Functional Evidence of Pulmonary Extracellular Vesicles in Infectious and Noninfectious Lung Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeedoo Lee et. al.PubMed 29997122
- Molecular medicine reports 18:723-7322018Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Jing Ran et. al.PubMed 29845205
- Cytometry. Part A : the journal of the Internation 93:448-4572018Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVera S Donnenberg et. al.PubMed 29498809
- Oncotarget 9:7522-75332018Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Xiu Guo et. al.PubMed 29484129
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04264
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
9 products for Alternative Product
2 products for Alternative Version
Target data
Function
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Alternative names
KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1
Recommended products
Mouse Monoclonal Cytokeratin 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04264
- Clone number
- C-11
- Purification technique
- Affinity purification Protein A
- Specificity
ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
- Concentration
- Purification notes
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
- Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
- Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
- Associated diseases and disorders
Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-intracellular-img86896.jpg "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.
Recommended conc. 0.5 μg/ml
Overlay with Isotype mouse IgG1 control.
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-intracellular-img446375.jpg "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Pan Cytokeratin Flow Cytometry (Intracellular) staining using mouse Anti-pan Cytokeratin antibody
ab52460 (concentration in sample 7.5 ug/ml, red-filled histogram) binds specifically to intracellular cytokeratins in permeabilized HeLa cells (upper panel), but does not stain permeabilized TF-1 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody (MOPC-21) under same conditions (concentration in sample 7.5 ug/ml, black-dashed histogram).
![Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-img446374.jpg "Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Pan Cytokeratin Flow Cytometry staining using mouse Anti-pan Cytokeratin antibody
Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using ab52460 (concentration in sample 7.5 ug/ml).
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