Mouse Monoclonal Cytokeratin 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.
Images
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-intracellular-img86896.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)")
Publications
Filter by
- Cancers 14:2022Modeling Colorectal Cancer Progression Reveals Niche-Dependent Clonal Selection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuria Vaquero-Siguero,Nikolai Schleussner,Julia Volk,Manuel Mastel,Jasmin Meier,Rene JackstadtPubMed 36077793
- Bio-protocol 10:e38222020Magnet-assisted Flow Cytometry of Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPreethi Korangath,Robert IvkovPubMed 33659474
- Theranostics 10:11026-110482020ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKok Suen Cheng et. al.PubMed 33042268
- Nature communications 10:54952019Senescent cell turnover slows with age providing an explanation for the Gompertz law.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmer Karin et. al.PubMed 31792199
- EBioMedicine 46:133-1492019CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukun Sun et. al.PubMed 31375425
- Cancer research 79:2962-29772019Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManjulata Singh et. al.PubMed 30952634
- Journal of immunology (Baltimore, Md. : 1950) 201:1500-15092018Functional Evidence of Pulmonary Extracellular Vesicles in Infectious and Noninfectious Lung Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeedoo Lee et. al.PubMed 29997122
- Molecular medicine reports 18:723-7322018Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Jing Ran et. al.PubMed 29845205
- Cytometry. Part A : the journal of the Internation 93:448-4572018Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVera S Donnenberg et. al.PubMed 29498809
- Oncotarget 9:7522-75332018Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Xiu Guo et. al.PubMed 29484129
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04264
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
10 products for Alternative Product
2 products for Alternative Version
Target data
Function
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Alternative names
67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1, KRTA, KRT1
Recommended products
Mouse Monoclonal Cytokeratin 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04264
- Clone number
C-11
- Purification technique
Affinity purification Protein A
- Specificity
ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
- Concentration
- Purification notes
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460), expandable thumbnail](https://content.abcam.com/products/images/pe-pan-cytokeratin-antibody-c-11-ab52460--flow-cytometry-intracellular-img86896.jpg "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.
Recommended conc. 0.5 μg/ml
Overlay with Isotype mouse IgG1 control.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com