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AB52460

PE Anti-pan Cytokeratin antibody [C-11]

5

(2 Reviews)

|

(18 Publications)

Mouse Monoclonal Cytokeratin 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

3 Images
Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

ab52460 (concentration in sample 7.5 ug/ml, red-filled histogram) binds specifically to intracellular cytokeratins in permeabilized HeLa cells (upper panel), but does not stain permeabilized TF-1 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody (MOPC-21) under same conditions (concentration in sample 7.5 ug/ml, black-dashed histogram).

Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using ab52460 (concentration in sample 7.5 ug/ml).

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.

Recommended conc. 0.5 μg/ml

Overlay with Isotype mouse IgG1 control.

  • Unconjugated

    Anti-pan Cytokeratin antibody [C-11]

  • 660 APC

    APC Anti-pan Cytokeratin antibody [C-11]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11]

  • Carrier free

    Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-pan Cytokeratin antibody [C-11]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-pan Cytokeratin antibody [C-11]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-pan Cytokeratin antibody [C-11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-11

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04264

Specificity

ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
See full target information KRT1
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed36077793

2022

Modeling Colorectal Cancer Progression Reveals Niche-Dependent Clonal Selection.

Applications

Unspecified application

Species

Unspecified reactive species

Nuria Vaquero-Siguero,Nikolai Schleussner,Julia Volk,Manuel Mastel,Jasmin Meier,Rene Jackstadt

Bio-protocol 10:e3822 PubMed33659474

2020

Magnet-assisted Flow Cytometry of Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Preethi Korangath,Robert Ivkov

Theranostics 10:11026-11048 PubMed33042268

2020

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kok Suen Cheng,Rongbin Pan,Huaping Pan,Binglin Li,Stephene Shadrack Meena,Huan Xing,Ying Jing Ng,Kaili Qin,Xuan Liao,Benson Kiprono Kosgei,Zhipeng Wang,Ray P S Han

Nature communications 10:5495 PubMed31792199

2019

Senescent cell turnover slows with age providing an explanation for the Gompertz law.

Applications

Unspecified application

Species

Unspecified reactive species

Omer Karin,Amit Agrawal,Ziv Porat,Valery Krizhanovsky,Uri Alon

EBioMedicine 46:133-149 PubMed31375425

2019

CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yukun Sun,Guangdong Wu,Kok Suen Cheng,Anqi Chen,Kuang Hong Neoh,Shuiyu Chen,Zhewen Tang,Poh Foong Lee,Menghua Dai,Ray P S Han

Cancer research 79:2962-2977 PubMed30952634

2019

Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Manjulata Singh,Xiao-Jun Tian,Vera S Donnenberg,Alan M Watson,JingYu Zhang,Laura P Stabile,Simon C Watkins,Jianhua Xing,Shilpa Sant

Journal of immunology (Baltimore, Md. : 1950) 201:1500-1509 PubMed29997122

2018

Functional Evidence of Pulmonary Extracellular Vesicles in Infectious and Noninfectious Lung Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Heedoo Lee,Duo Zhang,Debra L Laskin,Yang Jin

Molecular medicine reports 18:723-732 PubMed29845205

2018

Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Jing Ran,Yun Zeng,Shao-Chun Wang,Di-Si Zhang,Min Hong,Shao-You Li,Jian Dong,Ming-Xia Shi

Cytometry. Part A : the journal of the Internation 93:448-457 PubMed29498809

2018

Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Vera S Donnenberg,Jayce Jieming Zhang,Erika Moravcikova,Ernest Michael Meyer,Haihui Lu,Christian T Carson,Albert D Donnenberg

Oncotarget 9:7522-7533 PubMed29484129

2018

Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Xiu Guo,Kuang Hong Neoh,Xiao-Hong Chang,Yukun Sun,Hong-Yan Cheng,Xue Ye,Rui-Qiong Ma,Ray P S Han,Heng Cui
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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