AB52460

PE Anti-pan Cytokeratin antibody [C-11]

Name: PE Anti-pan Cytokeratin antibody [C-11] (ab52460) | Abcam
Brand: Abcam Limited
SKU: AB52460
Price: 605 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(22 Publications)

Mouse Monoclonal Cytokeratin 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

3 Images

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.

Recommended conc. 0.5 μg/ml

Overlay with Isotype mouse IgG1 control.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

ab52460 (concentration in sample 7.5 ug/ml, red-filled histogram) binds specifically to intracellular cytokeratins in permeabilized HeLa cells (upper panel), but does not stain permeabilized TF-1 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody (MOPC-21) under same conditions (concentration in sample 7.5 ug/ml, black-dashed histogram).

Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using ab52460 (concentration in sample 7.5 ug/ml).

Unconjugated

Anti-pan Cytokeratin antibody [C-11]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-pan Cytokeratin antibody [C-11]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-pan Cytokeratin antibody [C-11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-11

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04264

Specificity

ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.

Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1583271 PubMed40421296

2025

Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Qianren Zheng,Jie Chen,Yixun Huang,Weikai Chen,Dandan Cheng,Qianqian Jia,Meiqin Zhu,Youguo Liao,Qiulin He,Shunli Wu

NPJ precision oncology 9:144 PubMed40379789

2025

Multi-cancer detection of circulating tumor cells by targeting oncofetal chondroitin sulfate.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Løppke,Randi Ugleholdt,Christine F Secher,Nicolai T Sand,Joana Mujollari,Tobias Gustavsson,Robert Dagil,Thor G Theander,Ali Salanti,Kristoffer S Rohrberg,Mette Ø Agerbæk

Cancer immunology research 13:729-748 PubMed40249209

2025

Antitumor CD4+ T Helper 1 Cells Target and Control the Outgrowth of Disseminated Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesan Ramamoorthi,Marie Catherine Lee,Carly M Farrell,Colin Snyder,Saurabh K Garg,Amy L Aldrich,Vincent Lok,William Dominguez-Viqueira,Sy K Olson-Mcpeek,Marilin Rosa,Namrata Gautam,Shari Pilon-Thomas,Ling Cen,Krithika N Kodumudi,Doris Wiener,Thordur Oskarsson,Ana P Gomes,Robert A Gatenby,Brian J Czerniecki

Cancers 14: PubMed36077793

2022

Modeling Colorectal Cancer Progression Reveals Niche-Dependent Clonal Selection.

Applications

Unspecified application

Species

Unspecified reactive species

Nuria Vaquero-Siguero,Nikolai Schleussner,Julia Volk,Manuel Mastel,Jasmin Meier,Rene Jackstadt

Journal of experimental & clinical cancer research : CR 41:174 PubMed35562774

2022

A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Piao,Lingfeng Fu,Yuxin Wang,Yang Liu,Yue Wang,Xiangyu Meng,Dong Yang,Xiang Xiao,Jun Zhang

Bio-protocol 10:e3822 PubMed33659474

2020

Magnet-assisted Flow Cytometry of Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Preethi Korangath,Robert Ivkov

Theranostics 10:11026-11048 PubMed33042268

2020

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kok Suen Cheng,Rongbin Pan,Huaping Pan,Binglin Li,Stephene Shadrack Meena,Huan Xing,Ying Jing Ng,Kaili Qin,Xuan Liao,Benson Kiprono Kosgei,Zhipeng Wang,Ray P S Han

Nature communications 10:5495 PubMed31792199

2019

Senescent cell turnover slows with age providing an explanation for the Gompertz law.

Applications

Unspecified application

Species

Unspecified reactive species

Omer Karin,Amit Agrawal,Ziv Porat,Valery Krizhanovsky,Uri Alon

EBioMedicine 46:133-149 PubMed31375425

2019

CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yukun Sun,Guangdong Wu,Kok Suen Cheng,Anqi Chen,Kuang Hong Neoh,Shuiyu Chen,Zhewen Tang,Poh Foong Lee,Menghua Dai,Ray P S Han

Cancer research 79:2962-2977 PubMed30952634

2019

Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Manjulata Singh,Xiao-Jun Tian,Vera S Donnenberg,Alan M Watson,JingYu Zhang,Laura P Stabile,Simon C Watkins,Jianhua Xing,Shilpa Sant

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-pan Cytokeratin antibody [C-11]

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (AB52460)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise