JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB306194

PE Anti-PCK2 antibody [EPR14224] (C-terminal)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PCK2 antibody - conjugated to PE.

View Alternative Names

PEPCK2, PCK2, PEPCK-M, mtPCK2

  • 660 APC

    APC Anti-PCK2 antibody [EPR14224] (C-terminal)

  • HRP

    HRP Anti-PCK2 antibody [EPR14224] (C-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PCK2 antibody [EPR14224] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PCK2 antibody [EPR14224] - C-terminal

  • Unconjugated

    Anti-PCK2 antibody [EPR14224] - C-terminal

  • Carrier free

    Anti-PCK2 antibody [EPR14224] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14224

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoenolpyruvate carboxykinase 2 (PCK2) also known as mitochondrial PEPCK is an enzyme that plays an important role in gluconeogenesis. It catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the mitochondria. PCK2 has a molecular mass of approximately 69 kDa and is expressed mainly in tissues that require high energy production such as the liver and kidney. Despite its vital function in energy metabolism PCK2 is also present in other metabolic tissues.
Biological function summary

PCK2 contributes to the regulation of glucose homeostasis. It achieves this by being a part of the gluconeogenic pathway working in concert with cytosolic PCK1. PCK2 does not work alone; it integrates into a larger metabolic framework that includes various enzymes essential for converting different substrates into glucose. This enzyme is notably active during fasting states or periods of high energy demands such as intense exercise ensuring a continuous supply of glucose to maintain cellular functions.

Pathways

PCK2 plays an important role in the gluconeogenesis and TCA cycle. The gluconeogenesis pathway allows the liver and kidney to produce glucose from non-carbohydrate substrates which is integral during fasting and low-carbohydrate diet. It is functionally linked with other key enzymes like pyruvate carboxylase in converting pyruvate to glucose and also cooperates with malate dehydrogenase in the TCA cycle. This cooperation ensures efficient energy production and conversion balance in the cell.

PCK2 expression is linked to type 2 diabetes and certain cancers. In type 2 diabetes altered PCK2 activity impacts glucose production contributing to hyperglycemia. Studies have shown that its dysregulation may correlate with insulin resistance a characteristic feature of diabetes. In cancer particularly some forms of breast cancer the interplay between PCK2 and oncogenes like KRAS can affect tumor metabolism promoting the adaptation of cancer cells to the energy-demanding environment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed : 28955899). Can play an active role in glyceroneogenesis and gluconeogenesis (PubMed : 28955899).
See full target information PCK2
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB310193

Alexa Fluor® 647 Anti-UBA5 antibody [EPR11729]

RabMAb|Recombinant

  • 0
  • 0
Primary Antibodies

AB321341

Alexa Fluor® 750 Anti-SNRPD2 antibody [EPR16762]

Recombinant|RabMAb

  • 0
  • 0
Primary Antibodies

AB321412

Alexa Fluor® 750 Anti-HRPT2/Parafibromin antibody [EPR19927]

Recombinant|RabMAb

  • 0
  • 0
Proteins & Peptides

AB132473

Recombinant Human PCK2 protein

SDS-PAGE - Recombinant Human PCK2 protein (AB132473)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com