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AB233658

PE Anti-PCNA antibody [PC10]

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(1 Publication)

Mouse Monoclonal PCNA antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Pcna.

View Alternative Names

DNA sliding clamp PCNA, Cyclin, Proliferating cell nuclear antigen, PCNA

1 Images
Flow Cytometry (Intracellular) - PE Anti-PCNA antibody [PC10] (AB233658)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-PCNA antibody [PC10] (AB233658)

Intracellular staining of PCNA in K562 (human chronic myelogenous leukemia cell line from bone marrow) cells with ab233658.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PC10

Isotype

IgG2a

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Pcna.

P04961

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Alternative Products

Primary Antibodies

AB193964

Alexa Fluor® 647 Anti-PCNA antibody [EPR3821]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PCNA antibody [EPR3821] (AB193964)

  • 0
  • 0
Primary Antibodies

AB193965

HRP Anti-PCNA antibody [EPR3821]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-PCNA antibody [EPR3821] (AB193965)

  • 5
  • 3
Primary Antibodies

AB92552

Anti-PCNA antibody [EPR3821]

RabMAb|Lab Essentials|20ul selling size|Recombinant

Flow Cytometry (Intracellular) - Anti-PCNA antibody [EPR3821] (AB92552)

  • 5
  • 12
Primary Antibodies

AB218310

Anti-PCNA antibody [EPR3821] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PCNA antibody [EPR3821] - BSA and Azide free (AB218310)

  • 0
  • 0
Primary Antibodies

AB193963

Alexa Fluor® 488 Anti-PCNA antibody [EPR3821]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PCNA antibody [EPR3821] (AB193963)

  • 0
  • 0
Primary Antibodies

AB92729

Anti-PCNA antibody [EPR3822]

RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (AB92729)

  • 0
  • 0

Complementary Products

Assay Kits

AB196270

Human PCNA ELISA Kit

SimpleStep

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
Primary Antibodies

AB281847

Alexa Fluor® 488 Anti-Ki67 antibody [SP6]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [SP6] (AB281847)

  • 5
  • 1
Assay Kits

AB196270

Human PCNA ELISA Kit

SimpleStep

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Confers DNA tethering and processivity to DNA polymerases and other proteins (PubMed : 24695737, PubMed : 24939902, PubMed : 35585232). Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).
See full target information PCNA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of clinical cases 9:2181-2191 PubMed33869594

2021

Iguratimod promotes transformation of mononuclear macrophages in elderly patients with rheumatoid arthritis by nuclear factor-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Liu,Li-Ping Song,Rong-Bin Li,Le-Heng Feng,Hui Zhu
View all publications
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