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AB209962

PE Anti-PD-L1 antibody [28-8]

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(2 Publications)

Anti-PD-L1 antibody [28-8] - PE conjugated (ab209962) is a rabbit recombinant monoclonal antibody detecting PD-L1 in Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

1 Images
Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (AB209962)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-PD-L1 antibody [28-8] (AB209962)

Overlay histgram showing CHO (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab209962.

The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209962, 1/100 dilution) for 30 min at 4°C.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/42 bandpass filter.

  • 660 APC

    APC Anti-PD-L1 antibody [28-8]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PD-L1 antibody [28-8]

  • 519 FITC

    FITC Anti-PD-L1 antibody [28-8]

  • HRP

    HRP Anti-PD-L1 antibody [28-8]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PD-L1 antibody [28-8]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PD-L1 antibody [28-8]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PD-L1 antibody [28-8]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain

  • Biotin

    Biotin Anti-PD-L1 antibody [28-8] (Detector)

  • Unconjugated

    Anti-PD-L1 antibody [28-8]

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector)

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free

  • Carrier free

    Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

28-8

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478'>ab209478</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p><p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478'>ab209478</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p><p>The cellular localisation of this product has been verified in ICC/IF.</p>" } } }
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Product details

What is this antibody validated in?
PE Anti-PD-L1 antibody [28-8] (ab209962) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone 28-8 is also available pre-conjugated to a variety of labels for your convenience – Anti-PD-L1 PE [28-8] (ab209962).

Other related products
We have a range of other formats of antibody clone [28-8] also available for your convenience: ab205921, APC - ab206967, Carrier free - ab209889, Alexa Fluor® 488 - ab209959, Alexa Fluor® 647 - ab209960, HRP - ab209961, PE - ab209962, Alexa Fluor® 555 - ab213358, Alexa Fluor® 568 - ab213359, Alexa Fluor® 594 - ab213360, FITC - ab224027, Carrier free - ab228413, Carrier free - ab278010, Alexa Fluor® 647 - ab303724, Carrier free - ab307495, Biotin - ab322788

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70129 PubMed40766981

2025

Extracellular Vesicle Protein Panel Enables Early Lung Cancer Detection in a Large Clinical Cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Linxiao Han,Yuanlin Song,Lin Tong,Jiayuan Sun,Xiaoju Zhang,Shujing Chen,Ying Li,Ziqi Wang,Lei Gao,Qiaoliang Zhu,Yencheng Chao,Xiaocen Wang,Ge Zhang,Wensi Zhu,Ludan He,Jie Liu,Qin Wang,Zuoren Wu,Yuanyuan Ji,Chunxue Bai,Xiuzhen Lv,Jian Zhou

Molecular oncology 16:1347-1364 PubMed34687270

2021

Lung cancer cells expressing a shortened CDK16 3'UTR escape senescence through impaired miR-485-5p targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Jia,Baiyun Xie,Zhaozhao Zhao,Leihuan Huang,Gang Wei,Ting Ni
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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