AB47226

PE Anti-Perforin antibody [B-D48]

Name: PE Anti-Perforin antibody [B-D48] (ab47226) | Abcam
Brand: Abcam Limited
SKU: AB47226
Price: 565 USD
Availability: InStock

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(13 Publications)

Mouse Monoclonal Perforin antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRF1.

View Alternative Names

PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

1 Images

Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226)

Flow cytometric analysis of Perforin in YT-Indy cell line with ab47226.

This image was generated using the ascites version of the product.

Carrier free

Anti-Perforin antibody [B-D48] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-D48

Isotype

IgG1

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRF1.

P14222

Specificity

Recognizes human Perforin, a 70 kDa protein.

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Reactivity data

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Product details

This product was changed from ascites to tissue culture supernatant on 19^th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 1mL

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.09% Sodium azide Constituents: PBS, 5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.

Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 20889983, PubMed : 21037563, PubMed : 24558045, PubMed : 9058810, PubMed : 9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed : 9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 20889983, PubMed : 21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 20038786, PubMed : 20225066, PubMed : 24558045, PubMed : 32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed : 24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).

See full target information PRF1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:519 PubMed40671009

2025

CXCL12-targeting siRNA nanoparticles alleviate immunosuppression and inhibit tumor progression in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyao Zhang,Hong Jiang,Chengkuan Zhao,Yanli Lei,Shaojie Liu,Chengcheng Xu,Xiaoshan Chen,Danling Zheng,Xiaolong Wu,Xinyue Lin,Wang Chen,Yun Chen,Jianxiang Huang,XiaoLong Wei,Yihui Huang,Chaoxian Lin

Frontiers in cellular and infection microbiology 8:393 PubMed30510917

2018

Phenotypic and Functional Profiles of Antigen-Specific CD4 and CD8 T Cells Associated With Infection Control in Patients With Cutaneous Leishmaniasis.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Egui,Darién Ledesma,Elena Pérez-Antón,Andrés Montoya,Inmaculada Gómez,Sara María Robledo,Juan José Infante,Ivan Darío Vélez,Manuel C López,M Carmen Thomas

International journal of radiation oncology, biology, physics 103:320-334 PubMed30267761

2018

50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda G Herrera,Massimo Valerio,Dominik Berthold,Thomas Tawadros,Jean-Yves Meuwly,Veronique Vallet,Petra Baumgartner,Anne-Christine Thierry,Berardino De Bari,Patrice Jichlinski,Lana Kandalaft,George Coukos,Alexandre Harari,Jean Bourhis

PLoS neglected tropical diseases 12:e0006480 PubMed29750791

2018

Impact of benznidazole treatment on the functional response of Trypanosoma cruzi antigen-specific CD4+CD8+ T cells in chronic Chagas disease patients.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Pérez-Antón,Adriana Egui,M Carmen Thomas,Concepción J Puerta,John Mario González,Adriana Cuéllar,Manuel Segovia,Manuel Carlos López

Frontiers in microbiology 9:416 PubMed29599759

2018

Emergence of CD4+ and CD8+ Polyfunctional T Cell Responses Against Immunodominant Lytic and Latent EBV Antigens in Children With Primary EBV Infection.

Applications

Flow Cyt

Species

Human

Janice K P Lam,K F Hui,Raymond J Ning,X Q Xu,K H Chan,Alan K S Chiang

Scientific reports 7:11612 PubMed28912605

2017

Peripheral human CD4CD8 T lymphocytes exhibit a memory phenotype and enhanced responses to IL-2, IL-7 and IL-15.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Laure Clénet,François Gagnon,Ana Carmena Moratalla,Emilie C Viel,Nathalie Arbour

Journal of immunology (Baltimore, Md. : 1950) 198:3170-3180 PubMed28258194

2017

Antiparasitic Treatment Induces an Improved CD8 T Cell Response in Chronic Chagasic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jose Mateus,Elena Pérez-Antón,Paola Lasso,Adriana Egui,Nubia Roa,Bartolomé Carrilero,John M González,M Carmen Thomas,Concepción J Puerta,Manuel C López,Adriana Cuéllar

PloS one 11:e0150996 PubMed26974162

2016

Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Lasso,Lina Beltrán,Fanny Guzmán,Fernando Rosas,M Carmen Thomas,Manuel Carlos López,John Mario González,Adriana Cuéllar,Concepción J Puerta

PLoS pathogens 12:e1005349 PubMed26741490

2016

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Glen M Chew,Tsuyoshi Fujita,Gabriela M Webb,Benjamin J Burwitz,Helen L Wu,Jason S Reed,Katherine B Hammond,Kiera L Clayton,Naoto Ishii,Mohamed Abdel-Mohsen,Teri Liegler,Brooks I Mitchell,Frederick M Hecht,Mario Ostrowski,Cecilia M Shikuma,Scott G Hansen,Mark Maurer,Alan J Korman,Steven G Deeks,Jonah B Sacha,Lishomwa C Ndhlovu

Journal of immunology (Baltimore, Md. : 1950) 192:3730-9 PubMed24623134

2014

Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells.

Applications

Unspecified application

Species

Human

Werner J D Ouwendijk,Annemieke Geluk,Saskia L Smits,Sarah Getu,Albert D M E Osterhaus,Georges M G M Verjans

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Perforin antibody [B-D48]

Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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