Mouse Monoclonal Perforin antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRF1.
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![Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226), expandable thumbnail](https://content.abcam.com/products/images/pe-perforin-antibody-b-d48-ab47226--flow-cytometry-intracellular-img414608.gif "Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226)")
Publications
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- Frontiers in cellular and infection microbiology 8:3932018Phenotypic and Functional Profiles of Antigen-Specific CD4 and CD8 T Cells Associated With Infection Control in Patients With Cutaneous Leishmaniasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdriana Egui et. al.PubMed 30510917
- International journal of radiation oncology, biology, physics 103:320-334201850-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernanda G Herrera,Massimo Valerio,Dominik Berthold,Thomas Tawadros,Jean-Yves Meuwly,Veronique Vallet,Petra Baumgartner,Anne-Christine Thierry,Berardino De Bari,Patrice Jichlinski,Lana Kandalaft,George Coukos,Alexandre Harari,Jean BourhisPubMed 30267761
- PLoS neglected tropical diseases 12:e00064802018Impact of benznidazole treatment on the functional response of Trypanosoma cruzi antigen-specific CD4+CD8+ T cells in chronic Chagas disease patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Pérez-Antón et. al.PubMed 29750791
- Frontiers in microbiology 9:4162018Emergence of CD4+ and CD8+ Polyfunctional T Cell Responses Against Immunodominant Lytic and Latent EBV Antigens in Children With Primary EBV Infection.
- Scientific reports 7:116122017Peripheral human CD4CD8 T lymphocytes exhibit a memory phenotype and enhanced responses to IL-2, IL-7 and IL-15.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Laure Clénet et. al.PubMed 28912605
- Journal of immunology (Baltimore, Md. : 1950) 198:3170-31802017Antiparasitic Treatment Induces an Improved CD8 T Cell Response in Chronic Chagasic Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJose Mateus et. al.PubMed 28258194
- PloS one 11:e01509962016Promiscuous Recognition of a Trypanosoma cruzi CD8+ T Cell Epitope among HLA-A2, HLA-A24 and HLA-A1 Supertypes in Chagasic Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Lasso et. al.PubMed 26974162
- PLoS pathogens 12:e10053492016TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGlen M Chew et. al.PubMed 26741490
- Journal of immunology (Baltimore, Md. : 1950) 192:3730-92014Functional characterization of ocular-derived human alphaherpesvirus cross-reactive CD4 T cells.Applications:Unspecified applicationReactive species:HumanWerner J D Ouwendijk,Annemieke Geluk,Saskia L Smits,Sarah Getu,Albert D M E Osterhaus,Georges M G M VerjansPubMed 24623134
- PloS one 8:e774122013Activation associated ERK1/2 signaling impairments in CD8+ T cells co-localize with blunted polyclonal and HIV-1 specific effector functions in early untreated HIV-1 infection.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.09% Sodium azide
Constituents: 94.91% PBS, 5% BSA- Form
- Lyophilized
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PRF1. Database link P14222
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 10 μl to label 106 cells or 100 μl of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
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Target data
Function
Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:20889983, PubMed:21037563, PubMed:24558045, PubMed:9058810, PubMed:9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
Alternative names
PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein
Recommended products
Mouse Monoclonal Perforin antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRF1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.09% Sodium azide
Constituents: 94.91% PBS, 5% BSA- Form
- Lyophilized
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PRF1. Database link P14222
- Clone number
- B-D48
- Purity
- Tissue culture supernatant
- Specificity
Recognizes human Perforin, a 70 kDa protein.
- Light chain type
- kappa
- Reconstitution
- reconstitute with water at 1mL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.
- Biological function summary
Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.
- Pathways
Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.
- Associated diseases and disorders
Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (ab47226)
Flow cytometric analysis of Perforin in YT-Indy cell line with ab47226.
This image was generated using the ascites version of the product.
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