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AB322452

PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

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Rabbit Recombinant Monoclonal Pericentrin antibody - conjugated to PE. Centrosome marker. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.

View Alternative Names

KIAA0402, PCNT2, PCNT, Pericentrin, Kendrin, Pericentrin-B

5 Images
Flow Cytometry (Intracellular) - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Pericentrin - Centrosome Marker with ab322452 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunocytochemistry/ Immunofluorescence - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Pericentrin - Centrosome Marker with ab322452 at 1/50 (10.0 ug/ml) dilution.

Confocal image showing centrosome staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Pericentrin - Centrosome Marker with ab322452 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunocytochemistry/ Immunofluorescence - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Pericentrin - Centrosome Marker with ab322452 at 1/50 (10.0 ug/ml) dilution.

Confocal image showing centrosome staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry (Intracellular) - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB322452)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Pericentrin - Centrosome Marker with ab322452 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Pericentrin - Centrosome Marker antibody [EPR21987]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

  • Unconjugated

    Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

  • Carrier free

    Anti-Pericentrin antibody [EPR21987] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21987

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.
Biological function summary

Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.

Pathways

Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.

Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
See full target information PCNT
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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