Rabbit Recombinant Monoclonal Phospholipase C gamma 1/PLC-gamma-1 phospho Y783 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PLCG1 phospho Y783.
Images
![Flow Cytometry - PE Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278708), expandable thumbnail](https://content.abcam.com/products/images/pe-phospholipase-c-gamma-1-plc-gamma-1-phospho-y783-antibody-plcg1y783-c4-ab278708--flow-cytometry-img23332.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (AB278708)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human PLCG1 phospho Y783. The exact immunogen used to generate this antibody is proprietary information. Database link P19174
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µL for 106 Cells | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed:17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).
Alternative names
PLC1, PLCG1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, Phospholipase C-gamma-1, PLC-II, PLC-gamma-1
Recommended products
Rabbit Recombinant Monoclonal Phospholipase C gamma 1/PLC-gamma-1 phospho Y783 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PLCG1 phospho Y783.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human PLCG1 phospho Y783. The exact immunogen used to generate this antibody is proprietary information. Database link P19174
- Clone number
- PLCG1Y783-C4
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.
- Biological function summary
PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.
- Pathways
PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.
- Associated diseases and disorders
PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - PE Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (ab278708), expandable thumbnail](https://content.abcam.com/products/images/pe-phospholipase-c-gamma-1-plc-gamma-1-phospho-y783-antibody-plcg1y783-c4-ab278708--flow-cytometry-img23332.jpg "Flow Cytometry - PE Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (ab278708)")
Flow Cytometry - PE Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [PLCG1Y783-C4] (ab278708)
Flow cytometric analysis of Hela cells unstained imatinib treated (blue) or stained treated with imatinib (red) or with pervanadate (green) using ab278708.
Downloads
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- Visit the general protocols
- Visit troubleshooting
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