Rabbit Recombinant Monoclonal PRKAR1A antibody - conjugated to PE.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
cAMP-dependent protein kinase type I-beta regulatory subunit
PKR1, PRKAR1, TSE1, PRKAR1A, cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1
Rabbit Recombinant Monoclonal PRKAR1A antibody - conjugated to PE.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
PRKAR1A and PRKAR1B also known as protein kinase A regulatory subunit 1 alpha and beta are key components of the cAMP-dependent protein kinase (PKA) complex. PRKAR1A has a mass of approximately 43 kDa while PRKAR1B mass closely resembles it. Both PRKAR1A and PRKAR1B are expressed in various tissues with PRKAR1A having a significant presence in heart and adrenal tissues and PRKAR1B more prominent in the brain. Their role involves regulating the location and activity of the catalytic subunits of PKA by binding cAMP which causes the release and activation of catalytic subunits.
PRKAR1A and PRKAR1B participate in the regulation of PKA's activity which is important for cAMP signaling pathways. These proteins are part of the PKA holoenzyme complex functioning as inhibitory subunits. Upon binding cAMP they undergo a conformational change that releases PKA catalytic subunits allowing them to phosphorylate different substrates. This process affects cellular responses such as metabolism cell cycle progression and gene expression.
The PKA signaling pathway is among the major pathways involving PRKAR1A and PRKAR1B. The pathway is linked to G protein-coupled receptors (GPCR) and the subsequent release of cAMP. Another important pathway is the MAPK/ERK pathway where PKA modulates the activity of certain MAPK-related proteins affecting cell division and differentiation. PRKAR1A and PRKAR1B are involved with other proteins like PRKACB and PRKACA which are catalytic subunits of PKA.
PRKAR1A mutations associate with Carney complex a disorder characterized by multiple neoplasms. This connection results from PRKAR1A gene inactivation that leads to dysregulation of cAMP signaling. PRKAR1B on the other hand relates to neuropsychiatric disorders like schizophrenia where altered PKA signaling affects neurotransmitter pathways. Both PRKAR1A and PRKAR1B's involvement with regulatory proteins like PP1 which interacts with PKA highlights their significance in disease pathogenesis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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