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AB77228

PE Anti-PSMA antibody [GCP-05]

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(8 Publications)

Mouse Monoclonal PSMA antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FOLH1 aa 1 to C-terminus.

View Alternative Names

FOLH, NAALAD1, PSM, PSMA, GIG27, FOLH1, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I

1 Images
Flow Cytometry - PE Anti-PSMA antibody [GCP-05] (AB77228)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-PSMA antibody [GCP-05] (AB77228)

Surface staining (flow cytometry) of GCPII / PSMA using anti-GCPII (GCP-05) PE on LNCaP cell line.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GCP-05

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human FOLH1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q04609

Specificity

ab77228 recognizes extracellular domain of PSMA, expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
See full target information FOLH1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 127:2186-2197 PubMed36243890

2022

Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design.

Applications

Unspecified application

Species

Unspecified reactive species

Eske N Glud,Martin Rasmussen,Yonghui Zhang,Ole A Mandrup,Paul Vinu Salachan,Michael Borre,Karina Dalsgaard Sørensen,Kenneth A Howard

Biomarkers in medicine 14:1317-1327 PubMed32799659

2020

Study and detection of potential markers for predicting metastasis into lymph nodes in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis Papasotiriou,Panagiotis Apostolou,Dimitrios-Athanasios Ntanovasilis,Panagiotis Parsonidis,Daniar Osmonov,Klaus-Peter Jünemann

Oncoimmunology 8:e1672494 PubMed31741775

2019

Tumor-infiltrating lymphocytes from human prostate tumors reveal anti-tumor reactivity and potential for adoptive cell therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sharon Yunger,Assaf Bar El,Li-At Zeltzer,Eddie Fridman,Gil Raviv,Menachem Laufer,Jacob Schachter,Gal Markel,Orit Itzhaki,Michal J Besser

Science advances 5:eaaw5096 PubMed31281894

2019

Imaging CAR T cell therapy with PSMA-targeted positron emission tomography.

Applications

Unspecified application

Species

Unspecified reactive species

Il Minn,David J Huss,Hye-Hyun Ahn,Tamara M Chinn,Andrew Park,Jon Jones,Mary Brummet,Steven P Rowe,Polina Sysa-Shah,Yong Du,Hyam I Levitsky,Martin G Pomper

Neuroscience 144:1361-72 PubMed17150306

2006

Expression of glutamate carboxypeptidase II in human brain.

Applications

Unspecified application

Species

Unspecified reactive species

P Sácha,J Zámecník,C Barinka,K Hlouchová,A Vícha,P Mlcochová,I Hilgert,T Eckschlager,J Konvalinka

European journal of biochemistry 271:2782-90 PubMed15206943

2004

Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding.

Applications

Unspecified application

Species

Unspecified reactive species

Cyril Barinka,Petra Mlcochová,Pavel Sácha,Ivan Hilgert,Pavel Majer,Barbara S Slusher,Václav Horejsí,Jan Konvalinka

Protein science : a publication of the Protein Society 13:1627-35 PubMed15152093

2004

Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Cyril Barinka,Pavel Sácha,Jan Sklenár,Petr Man,Karel Bezouska,Barbara S Slusher,Jan Konvalinka

Journal of neurochemistry 80:477-87 PubMed11905994

2002

Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II.

Applications

Unspecified application

Species

Unspecified reactive species

Cyril Barinka,Markéta Rinnová,Pavel Sácha,Camilo Rojas,Pavel Majer,Barbara S Slusher,Jan Konvalinka
View all publications
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