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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with samples. Cited in 4 publications.
IgG
Rabbit
PE
Ex: 480;565nm, Em: 578nm
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1/100 | Notes This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
Application Flow Cyt (Intra) | Reactivity Reacts | Dilution info - | Notes Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. |
PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with samples. Cited in 4 publications.
IgG
Rabbit
PE
Ex: 480;565nm, Em: 578nm
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR25A
Affinity purification Protein A
Blue Ice
+4°C
Upon delivery aliquot
Store in the dark
KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometry overlay histogram showing HeLa cells stained with ab209446 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% Methanol for 30 min at -20°C. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209446) (1x106 in 100μL at 0.01μg/mL, 0.1μg/mL, 1μg/mL (1/50000, 1/5000, 1/500)) for 30 min at 22°C.
Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 100% methanol (5 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/100 dilution (showing no signal) and ab190573, Rabbit monoclonal to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10min).
Flow cytometry overlay histogram showing Isotype (Left), HEK293T transfected with myc-His-DLL3 (Middle), and HEK293T cells transfected with an empty expression vector containing a myc-His-tag® (Right). The cells were fixed with 4% formaldehyde and then permeabilised with 90% methanol. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab305808) (1x 106 in 100μl at 1μg/ml (1/500)) for 30min at 22°C. The cells were also incubated with Alexa Fluor®647 anti-myc antibody.
Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/16 bandpass filter.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (mouse atrial muscle cell) cells labelling Troponin T with ab322129 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab318309 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab318309 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 µl at 0.04 μg/ml (1/12)) for 30min on ice. The cells were simultaneously stained with c-Kit.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live cells.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (rat skeletal muscle myoblast) cells labelling Troponin T with ab322129 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Flow cytometry overlay histogram showing wild-type A549 (green line) and ALDH1A1 knockout A549 cells stained with ab209437 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209437) (1x 106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min at 22°C.
Isotype control antibody Rabbit IgG monoclonal Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, ALDH1A1 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in wild-type A549 fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry staining of C57 BL/6 mouse splenocytes (top) or C57 BL/6 mouse splenocytes treated with PMA/TPA 80nM, Ionomycin 1.34uM, Brefeldin A 1ug/mL , 18 Hours (bottom) with ab318310 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab318310 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 µl at 0.2 μg/ml (1/2)) for 30min at 22°C. The cells were simultaneously stained with CD4.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live cells.
Flow cytometry overlay histogram showing Neuro-2A cells stained with ab314256 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314256) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in Neuro-2A Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry staining of Human PBMCs (top) or PBMCs treated with 1μg/ml LPS and 1μg/ml Brefeldin A for 18h (bottom), with ab317072 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab317072 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 µl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD14.
Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/16 bandpass filter. Events were gated on viable cells.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 µl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD11b.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry overlay histogram showing left THP-1 positive cells and right negative Jurkat stained with ab314295 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314295) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 µl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab315145 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315145 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD56.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab315144 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315144 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD49b.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry staining of C57 BL/6 mouse splenocytes with ab315144 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315144 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD19.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry overlay histogram showing wild-type HeLa (green line) and L1CAM knockout HeLa stained with ab315143 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab315143) (1x 106in 100µl at 0.2 µg/ml (1/2500)) for 30min on ice.
Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line, L1CAM knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314947 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314947 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106in 100 µl at 0.04 µg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD14.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry overlay histogram showing Ms Thymocytes stained with ab314416 (red line). The cells were incubated in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314416) (1x 10⁶ in 100μl at 5.0 μg/ml (1/100)) for 30min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing HeLa cells stained with ab314402 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314402) (1x 10⁶ in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in HeLa Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry overlay histogram showing left K-562 positive cells and right negative PC3 stained with ab314270 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314270) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in K-562 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry overlay histogram showing left Raw264.7 positive cells and right negative Neuro-2A stained with ab314259 (red line). The cells were incubated in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314259) (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice.
Isotype control antibody (black line) was PE Rat IgG2a, monoclonal [2A3] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing left HEK293 positive cells and right negative Jurkat stained with ab314298 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314298) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing wild-type HEK293T (green line) and cd276 knockout HEK293T stained with ab314419 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314419) (1x 10⁶ in 100μl at 1.0 μg/ml (1/500)) for 30min on ice.
Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type HEK293T - black line, cd276 knockout HEK293T - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing left HeLa positive cells and right negative THP-1 stained with ab314417 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314417) (1x 10⁶ in 100μl at 1.0 μg/ml (1/500)) for 30min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing left U87-MG positive cells and right negative HUVEC stained with ab314406 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314406) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing HeLa cells stained with ab316178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab316178) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry overlay histogram showing T-47D cells stained with ab225508 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab225508) (1x 106 in 100µl at a 1/12500 dilution for 30 min at 22° C.
Isotype control antibody (black line) was Rabbit IgG monoclonal Phycoerythrin Isotype Control ab209478 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in T-47D Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry overlay histogram showing left Bend.3 positive cells and right negative NIH/3T3 stained with ab314294 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314294) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry overlay histogram showing HeLa cells stained with ab314286 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314286) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry overlay histogram showing left Raji positive cells and right negative A-375 stained with ab314248 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314248) (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in Raji Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Flow cytometry overlay histogram showing left THP-1 positive cells and right negative HCT116 stained with ab314261 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314261) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314269 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 22°C in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314269 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.0016 μg/ml (1/312500)) for 30 min at 22°C . The cells were simultaneously stained with CD19.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314260 (right) or PE Mouse IgG1 [B11/6] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314260 or PE Mouse IgG1 [B11/6] - Isotype Control (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD19.
Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.
Flow cytometry overlay histogram showing HeLa cells stained with ab314253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314253) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
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