PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

40 product images

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow cytometry overlay histogram showing HeLa cells stained with PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab209446 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% Methanol for 30 min at -20°C. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab209446) (1x10⁶ in 100μL at 0.01μg/mL, 0.1μg/mL, 1μg/mL (1/50000, 1/5000, 1/500)) for 30 min at 22°C.
  

  Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

• 

  Immunocytochemistry/ Immunofluorescence - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 100% methanol (5 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/100 dilution (showing no signal) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10min).

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of mouse splenocytes/ Right using rabbit Anti- antibody

  Flow cytometric analysis of mouse splenocytes labelling CD35 + CD21 with PE Anti-CD21+CD35 antibody [EPR27417-513] ab324033 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells are co-stained with CD3 conjugated to Alexa Fluor®488.
  
  Gated on viable cell.

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry (Intracellular) staining of A549 WT using rabbit Anti- antibody

  Flow cytometry overlay histogram showing wild-type A549 (green line) and TGOLN2 knockout A549 stained with PE Anti-TGN46 antibody [EPR24770-16] - Golgi Marker ab313945 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (PE Anti-TGN46 antibody [EPR24770-16] - Golgi Marker ab313945) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30 min at 22°C.
  
  Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, TGOLN2 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
  
  Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry (Intracellular) staining of Neuro-2A cells using rabbit Anti- antibody

  Flow cytometry overlay histogram showing Neuro-2A cells stained with PE Anti-LAMP1 antibody [EPR21026] - Lysosome Marker ab314256 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (PE Anti-LAMP1 antibody [EPR21026] - Lysosome Marker ab314256) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.
  
  Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
  
  Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
  
  This antibody gave a positive signal in Neuro-2A Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry (Intracellular) staining using rabbit Anti- antibody

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector (Middle) / 293T cells transfected with an empty expression vector (Right) cells labelling CD27 with PE Anti-CD27 antibody [EPR27428-9] ab324175 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry (Intracellular) staining using rabbit Anti- antibody

  Overlay histogram showing HeLa cells untreated (magenta line) and HeLa cells treated with Chloroquine, 50μM, 24 hours, (green line) stained with PE Anti-SQSTM1 / p62 antibody [EPR18351] - Autophagosome Marker ab225454. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (PE Anti-SQSTM1 / p62 antibody [EPR18351] - Autophagosome Marker ab225454, 1µg/ml) for 30 min at 22°C.
  

  Isotype control antibody (HeLa cells untreated - black line, HeLa cells treated - grey line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody.

  Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Rat PBMC (Rat peripheral blood mononuclear cell)/ Right using rabbit Anti- antibody

  Flow cytometric analysis of Rat PBMC (Rat peripheral blood mononuclear cell) cells labelling CD27 with PE Anti-CD27 antibody [EPR27428-9] ab324175 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse PBMCs (mouse primary peripheral blood mononuclear cells)/ Right using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD27 with PE Anti-CD27 antibody [EPR27428-9] ab324175 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse PBMCs (mouse primary peripheral blood mononuclear cells)/ Right using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD27 with PE Anti-CD27 antibody [EPR27428-9] ab324175 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell)/ Right using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with PE Anti-CD27 antibody [EPR27428-9] ab324175 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell)/ Right using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with PE Anti-CD27 antibody [EPR27428-9] ab324175 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse PBMC (mouse peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD36 with PE Anti-CD36 antibody [EPR27433-40] ab323779 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD36 with PE Anti-CD36 antibody [EPR27433-40] ab323779 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: NIH/3T3 (PMID: 7543064).
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse bone marrow using rabbit Anti- antibody

  Flow cytometric analysis of Mouse bone marrow cells labelling CD34 with PE Anti-CD34 antibody [EPR27431-71] ab323757 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Cells are co-stained with CD117 conjugated to APC-eFluor 780. (PMID: 23295606)

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CD13 with PE Anti-CD13 antibody [EPR27472-288] ab323783 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Negative control: EL4 (PMID: 8103749).

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse spleen cell using rabbit Anti- antibody

  Flow cytometric analysis of Mouse spleen cell cells labelling CD13 with PE Anti-CD13 antibody [EPR27472-288] ab323783 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®647.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse spleen cell using rabbit Anti- antibody

  Flow cytometric analysis of Mouse spleen cell cells labelling CD13 with PE Anti-CD13 antibody [EPR27472-288] ab323783 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD11c conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse peripheral blood mononuclear cell (PBMC) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD45 with PE Anti-CD45 antibody [EPR28934-536] ab323886 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of HL-1 (mouse atrial muscle cell, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CD45 with PE Anti-CD45 antibody [EPR28934-536] ab323886 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Low expression: HL-1.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of C2C12 (mouse myoblast, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD45 with PE Anti-CD45 antibody [EPR28934-536] ab323886 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Low expression: C2C12.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse bone marrow using rabbit Anti- antibody

  Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with PE Anti-CCR3 antibody [EPR27419-30] ab323784 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD3 cojugated to Alexa Fluor®488.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse bone marrow using rabbit Anti- antibody

  Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with PE Anti-CCR3 antibody [EPR27419-30] ab323784 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of A20 (mouse reticulum sarcoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CCR3 with PE Anti-CCR3 antibody [EPR27419-30] ab323784 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Low expression: A20.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD63 with PE Anti-CD63 antibody [EPR22458-280] ab322527 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Cells were stained with anti-CD14 conjugated to Alexa Fluor® 647.

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry (Intracellular) staining of SK-MEL-28 (human malignant melanoma cell line) using rabbit Anti- antibody

  Flow cytometric analysis of SK-MEL-28 (human malignant melanoma cell line) cells labelling CD63 with PE Anti-CD63 antibody [EPR22458-280] ab322527 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Left panel: No fixation/permeabilization; Gated on viable cells for surface CD63 staining.
  
  Right panel: 4% paraformadehyde fixation / 90% methanol permeabilization.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse thymocytes using rabbit Anti- antibody

  Flow cytometric analysis of Mouse thymocytes cells labelling CD147 with PE Anti-CD147 antibody [EPR18008-8] ab323725 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of C57 BL/6 mouse splenocytes using rabbit Anti- antibody

  Flow cytometry staining of C57 BL/6 mouse splenocytes with PE Anti-VCAM1 antibody [EPR27027-79] ab323936 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody PE Anti-VCAM1 antibody [EPR27027-79] ab323936 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD3.

  Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometry overlay histogram showing left Hut-78 positive cells and right negative SK-OV-3 stained with PE Anti-VCAM1 antibody [EPR27027-79] ab323936 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (PE Anti-VCAM1 antibody [EPR27027-79] ab323936) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

  Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Mouse PBMC (mouse peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD36 with PE Anti-CD36 antibody [EPR27433-40] ab323779 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with PE Anti-CD2 antibody [EPR27426-17] ab323756 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD19 conjugated to Alexa Fluor® 647.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling FCRL5 with PE Anti-FCRL5 antibody [EPR26948-187] ab323752 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti human CD3 conjugated to Brilliant Violet 421.
  
  Gated on viable Lymphocytes.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of Hepa1-6 (mouse hepatoma epithelial cell, Left) / C2C12 (mouse myoblast, Right) cells labelling Podoplanin with PE Anti-Podoplanin antibody [EPR27193-535] ab323754 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Low expression: Hepa1-6.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD2 with PE Anti-CD2 antibody [EPR27426-17] ab323756 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Raji (PMID: 32808354).
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining using rabbit Anti- antibody

  Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling CD34 with PE Anti-CD34 antibody [EPR27431-71] ab323757 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Neuro-2a.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD2 with PE Anti-CD2 antibody [EPR27426-17] ab323756 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells are co-stained with CD56 conjugated to Brilliant Violet 421.
  
  Gated on viable cells.

• 

  Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti- antibody

  Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with PE Anti-CD300a antibody [EPR27995-84] ab323732 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Cells were co-stained with anti human CD19 conjugated to Alexa Fluor?647.
  
  Gated on viable cells.

• 

  Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  Flow Cytometry (Intracellular) staining using rabbit Anti- antibody

  Flow cytometry overlay histogram showing left SK-N-SH positive cells and right negative A-498 stained with PE Anti-Nestin antibody [EPR1301(2)] - Neural Stem Cell Marker ab322987 (red line). The cells were fixed with 8% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (PE Anti-Nestin antibody [EPR1301(2)] - Neural Stem Cell Marker ab322987) (1x 10⁶ in 100 ul at 0.0016 ug/ml (1/312500)) for 30min at 22°C.

  Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.