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Rabbit Recombinant Monoclonal Serine/threonine-protein kinase 4/MST-1 antibody - conjugated to PE.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Target data

Function

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Serine/threonine-protein kinase 4/MST-1 antibody - conjugated to PE.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1465Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Serine/threonine-protein kinase 4 (MST-1) also known as STK4 is a 52 kDa enzyme that plays an important role in cellular signaling processes. This kinase ubiquitously expresses in many tissues including liver kidney and heart. MST-1 functions as a part of a complex facilitating its catalytic activity by activating downstream substrates. The alternative name for this protein is mammalian STE20-like protein kinase 1 linking MST-1 to a family of serine/threonine kinases.

Biological function summary

This kinase orchestrates various cellular processes mainly through regulation of apoptosis and cell stress responses. MST-1 interacts with other signaling molecules in cells forming part of larger multi-protein complexes. These interactions allow MST-1 to act as a molecular switch effectively controlling the balance between cell survival and death. The cellular localization and interaction partners help diversify its biological roles.

Pathways

MST-1 predominantly influences the Hippo signaling pathway—a pathway significant in controlling organ size and suppressing cancer. In this pathway MST-1 phosphorylates and activates LATS1/2 kinases creating an endpoint effect on cell proliferation and apoptosis. MST-1 also participates in the oxidative stress response pathway where it shows interaction with FoxO transcription factors leading to modifications in gene expression profiles.

Associated diseases and disorders

The dysregulation of MST-1 is linked to cancer and cardiovascular diseases. In cancer the Hippo pathway's consistent activation by MST-1 helps modulate uncontrolled cell division while in cardiovascular diseases MST-1 plays a role by driving cardiomyocyte apoptosis. MST-1’s interplay with FoxO transcription factors also highlights its contribution to these diseases by reinforcing stress response and DNA repair mechanisms.

Product promise

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