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AB240998

PE Anti-SIGLEC5 antibody [1A5]

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(1 Publication)

Mouse Monoclonal SIGLEC5 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SIGLEC5.

View Alternative Names

CD170, CD33L2, OBBP2, SIGLEC5, Sialic acid-binding Ig-like lectin 5, Siglec-5, CD33 antigen-like 2, Obesity-binding protein 2, OB-BP2, OB-binding protein 2

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Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (AB240998)
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Supplier Data

Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (AB240998)

Flow cytometric analysis of human peripheral blood cells labeling SIGLEC5 with ab240998. Surface staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A5

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SIGLEC5. The exact immunogen used to generate this antibody is proprietary information.

O15389

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC5 also known as CD170 is a sialic acid-binding Ig-like lectin with a molecular mass of approximately 96 kDa. This protein plays an important role in immune cell signaling by recognizing sialylated glycans on the surface of cells. SIGLEC5 is mainly expressed on immune cells such as neutrophils monocytes and macrophages. It belongs to the lectin family of proteins and can modulate immune responses and communication between cells.
Biological function summary

SIGLEC5 functions by mediating cell-cell interactions and transmitting inhibitory signals that regulate immune responses. It binds to sialic acid-containing glycoproteins which can help modulate the threshold for cell activation by transmitting signals through its immunoreceptor tyrosine-based inhibitory motif (ITIM). SIGLEC5 is not part of a large complex but interacts with other molecules to exert its function in maintaining immune cell balance.

Pathways

SIGLEC5 plays a role in immune signaling pathways by maintaining cell homeostasis. It contributes to the regulation of the immune response by modulating inhibitory signaling pathways. It is associated with proteins like Src family kinases which phosphorylate the ITIM motif to initiate downstream signaling cascades. Additionally its interaction with sialic acids allows modulation of the innate immune response to various stimuli.

SIGLEC5 has been linked to autoimmune diseases and infections like bacterial sepsis. In autoimmune conditions the dysregulation of SIGLEC5-associated pathways can lead to improper immune cell activation and tissue damage. In the context of bacterial sepsis SIGLEC5 assists in dampening excessive immune responses and limiting inflammation. SIGLEC5 connects with proteins like DAP12 where aberrations could also influence disease progressions making it a target of interest for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
See full target information SIGLEC5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 24:1012-1023 PubMed34141456

2021

lncRNA ILF3-AS1 promotes proliferation and metastasis of colorectal cancer cells by recruiting histone methylase EZH2.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Hong,Shiquan Li,Miaomiao Bi,Haiyao Yu,Zhenkun Yan,Tao Liu,Helei Wang
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