JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213195

PE Anti-SIRP alpha antibody [EPR16264]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SIRP alpha antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3

1 Images
Flow Cytometry (Intracellular) - PE Anti-SIRP alpha antibody [EPR16264] (AB213195)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-SIRP alpha antibody [EPR16264] (AB213195)

Overlay histogram showing PC12 cells stained with ab213195 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213195, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in PC12 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16264

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB8120

Anti-SIRP alpha antibody

Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody (AB8120)

  • 5
  • 2
Primary Antibodies

AB53721

Anti-SIRP alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (AB53721)

  • 5
  • 1
Primary Antibodies

AB34076

FITC Anti-SIRP alpha antibody [OX-41]

  • 0
  • 0
Primary Antibodies

AB34086

Anti-SIRP alpha antibody [ED9]

  • 0
  • 0
Primary Antibodies

AB33987

Biotin Anti-SIRP alpha antibody [OX-41]

  • 0
  • 0
Primary Antibodies

AB23772

FITC Anti-SIRP alpha antibody [BL1H7]

  • 0
  • 0
Primary Antibodies

AB232765

Anti-SIRP alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (AB232765)

  • 0
  • 0
Primary Antibodies

AB260039

Anti-SIRP alpha antibody [EPR22930-163]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Flow Cytometry - Anti-SIRP alpha antibody [EPR22930-163] (AB260039)

  • 5
  • 1
Primary Antibodies

AB267401

Anti-SIRP alpha antibody [EPR22930-163] - BSA and Azide free

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Flow Cytometry - Anti-SIRP alpha antibody [EPR22930-163] - BSA and Azide free (AB267401)

  • 0
  • 0
Primary Antibodies

AB116254

Anti-SIRP alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (AB116254)

  • 0
  • 0
Primary Antibodies

AB95644

Biotin Anti-SIRP alpha antibody [P84]

Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (AB95644)

  • 5
  • 1

Complementary Products

Assay Kits

AB272477

Human SIRP alpha ELISA Kit (CD172a)

Recombinant|SimpleStep

ELISA - Human SIRP alpha ELISA Kit (CD172a) (AB272477)

  • 0
  • 0
Primary Antibodies

AB281764

Alexa Fluor® 555 Anti-CD16 antibody [EPR16784]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD16 antibody [EPR16784] (AB281764)

  • 0
  • 0
Proteins & Peptides

AB322096

Recombinant Human SIRP alpha Protein Standard (His tag)

Sandwich ELISA - Recombinant Human SIRP alpha Protein Standard (His tag) (AB322096)

  • 0
  • 0
Primary Antibodies

AB317478

APC Anti-CD62L antibody [EPR23565-109]

Recombinant|RabMAb

Flow Cytometry - APC Anti-CD62L antibody [EPR23565-109] (AB317478)

  • 4
  • 1
Primary Antibodies

AB201742

Alexa Fluor® 594 Anti-NCAM1 antibody [EPR2566]

RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 594 Anti-NCAM1 antibody [EPR2566] (AB201742)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.
Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed : 24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed : 24511121).
See full target information SIRPA
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:4713-4732.e19 PubMed38968937

2024

Progestogen-driven B7-H4 contributes to onco-fetal immune tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Yu,Yijian Yan,Shasha Li,Ying Xu,Abhijit Parolia,Syed Rizvi,Weichao Wang,Yiwen Zhai,Rongxin Xiao,Xiong Li,Peng Liao,Jiajia Zhou,Karolina Okla,Heng Lin,Xun Lin,Sara Grove,Shuang Wei,Linda Vatan,Jiantao Hu,Justyna Szumilo,Jan Kotarski,Zachary T Freeman,Stephanie Skala,Max Wicha,Kathleen R Cho,Arul M Chinnaiyan,Samantha Schon,Fei Wen,Ilona Kryczek,Shaomeng Wang,Lieping Chen,Weiping Zou
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com