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AB324287

PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86]

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Rabbit Recombinant Monoclonal SLC38A5 / SNAT5 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), Flow Cyt and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

JM24, SN2, SNAT5, PP7194, SLC38A5, Sodium-coupled neutral amino acid transporter 5, Solute carrier family 38 member 5, System N transporter 2

3 Images
Flow Cytometry - PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (AB324287)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (AB324287)

Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell) (Right) / A549 (human lung carcinoma epithelial cell) (Left) cells labelling SLC38A5 / SNAT5 with ab324287 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Low expression : A549.
Gated on viable cells.

Flow Cytometry - PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (AB324287)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (AB324287)

Flow cytometric analysis of 143B (human osteosarcoma cell, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling SLC38A5 / SNAT5 with ab324287 at 1/50 dilution (1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Low expression : 143B (PMID : 27129276).
Gated on viable cells.

Flow Cytometry - PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (AB324287)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] (AB324287)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a human SLC38A5 expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling SLC38A5 / SNAT5 with ab324287 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Live cells were surface stained with our antibody, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

  • Carrier free

    Anti-SLC38A5 / SNAT5 antibody [EPR27109-86] - BSA and Azide free

  • Unconjugated

    Anti-SLC38A5 / SNAT5 antibody [EPR27109-86]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27109-86

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell line - Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50000", "FlowCytIntra-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Symporter that cotransports neutral amino acids and sodium ions, coupled to an H(+) antiporter activity (PubMed : 11243884). Releases L-glutamine and glycine from astroglial cells and may participate in the glutamate/GABA-L-glutamine cycle and the NMDA receptors activation (By similarity). In addition, contributes significantly to L-glutamine uptake in retina, namely in ganglion and Mueller cells therefore, participates in the retinal glutamate-glutamine cycle (By similarity). The transport activity is pH sensitive and Li(+) tolerant (PubMed : 11243884). Moreover functions in both direction and is associated with large uncoupled fluxes of protons (By similarity). The transport is electroneutral coupled to the cotransport of 1 Na(+) and the antiport of 1 H(+) (By similarity). May have a particular importance for modulation of net hepatic glutamine flux (By similarity).
See full target information SLC38A5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com