JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317888

PE Anti-SLFN12 antibody [EPR20904-32]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SLFN12 antibody - conjugated to PE. Suitable for Target Binding Affinity, Antibody Labelling, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Ribonuclease SLFN12, Schlafen family member 12, SLFN12

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20904-32

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Target Binding Affinity, Flow Cyt (Intra), Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "TargetBindingAffinity" : {"fullname" : "Target Binding Affinity", "shortname":"Target Binding Affinity"}, "AntibodyLabelling" : {"fullname" : "Antibody Labelling", "shortname":"Antibody Labelling"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "TargetBindingAffinity-species-checked": "guaranteed", "TargetBindingAffinity-species-dilution-info": "", "TargetBindingAffinity-species-notes": "", "AntibodyLabelling-species-checked": "guaranteed", "AntibodyLabelling-species-dilution-info": "", "AntibodyLabelling-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLFN12 also known as Schlafen family member 12 is a protein that plays a role in protein synthesis regulation and innate immunity. It carries a mass of approximately 65 kDa. The protein shows expression in multiple human tissues including the colon small intestine and prostate. SLFN12 contains a notable AAA+ ATPase domain which suggests involvement in processes requiring energy such as molecular chaperone activity. Researchers have studied its expression pattern noting its presence at the cellular level which hints at diverse functional implications across various cells.
Biological function summary

SLFN12 modulates the differentiation of cells and appears to play a role in immune response. It does not form part of a large protein complex. It functions independently to exert effects specifically influencing maturation of specific immune cells and contributing to maintaining cellular homeostasis. Its role in differentiation makes it significant in balancing immune responses ensuring cells reach necessary maturity to perform their function in defense mechanisms.

Pathways

Studies highlight that SLFN12 contributes to the regulation of protein synthesis and the immune response pathway. In the synthesis pathway it potentially interacts with ribosomal proteins to regulate production of proteins modulating cell homoeostasis and growth. Meanwhile its involvement in the immune response pathway shows interaction with other SLFN family proteins altering cellular responses to microbial infections. Research also suggests it operates alongside proteins like SLFN11 to ensure effective cellular defense against pathogenic attacks.

Researchers have associated SLFN12 with inflammatory bowel disease and certain types of cancer such as prostate cancer. Its expression patterns in inflamed tissue suggest that SLFN12 may mediate inflammatory responses exacerbating or alleviating the condition. In prostate cancer SLFN12 seems to interact with the androgen receptor pathway potentially influencing cancer cell proliferation. Understanding these interactions and their pathological consequences will provide insights into its role in disease mechanisms and offer perspectives for therapeutics targeting SLFN12-mediated pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ribonuclease which is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling (PubMed : 31420216, PubMed : 34272366, PubMed : 34707099, PubMed : 35104454). May play a role in cell differentiation (PubMed : 30045019).
See full target information SLFN12
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com