AB209299

PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

1 Images

Flow Cytometry (Intracellular) - PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB209299)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB209299)

Overlay histogram showing HeLa cells stained with ab209299 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209299, 1/2500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
Biotin

Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
HRP

HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
660 APC

APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
Unconjugated

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
Carrier free

Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1845Y

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes an intracellular epitope.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Tilapia": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:1094124 PubMed36699014

2023

The tendon interfascicular basement membrane provides a vascular niche for CD146+ cell subpopulations.

Applications

Unspecified application

Species

Unspecified reactive species

Neil Marr,Danae E Zamboulis,Dirk Werling,Alessandro A Felder,Jayesh Dudhia,Andrew A Pitsillides,Chavaunne T Thorpe

Cell metabolism 34:857-873.e9 PubMed35561674

2022

A diabetic milieu increases ACE2 expression and cellular susceptibility to SARS-CoV-2 infections in human kidney organoids and patient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Garreta,Patricia Prado,Megan L Stanifer,Vanessa Monteil,Andrés Marco,Asier Ullate-Agote,Daniel Moya-Rull,Amaia Vilas-Zornoza,Carolina Tarantino,Juan Pablo Romero,Gustav Jonsson,Roger Oria,Alexandra Leopoldi,Astrid Hagelkruys,Maria Gallo,Federico González,Pere Domingo-Pedrol,Aleix Gavaldà,Carmen Hurtado Del Pozo,Omar Hasan Ali,Pedro Ventura-Aguiar,Josep María Campistol,Felipe Prosper,Ali Mirazimi,Steeve Boulant,Josef M Penninger,Nuria Montserrat

Cell 181:905-913.e7 PubMed32333836

2020

Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Monteil,Hyesoo Kwon,Patricia Prado,Astrid Hagelkrüys,Reiner A Wimmer,Martin Stahl,Alexandra Leopoldi,Elena Garreta,Carmen Hurtado Del Pozo,Felipe Prosper,Juan Pablo Romero,Gerald Wirnsberger,Haibo Zhang,Arthur S Slutsky,Ryan Conder,Nuria Montserrat,Ali Mirazimi,Josef M Penninger

Nature materials 18:397-405 PubMed30778227

2019

Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Garreta,Patricia Prado,Carolina Tarantino,Roger Oria,Lucia Fanlo,Elisa Martí,Dobryna Zalvidea,Xavier Trepat,Pere Roca-Cusachs,Aleix Gavaldà-Navarro,Luca Cozzuto,Josep M Campistol,Juan Carlos Izpisúa Belmonte,Carmen Hurtado Del Pozo,Nuria Montserrat

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

Flow Cytometry (Intracellular) - PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB209299)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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