Rabbit Monoclonal SOX10 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - PE Anti-SOX10 antibody [SP267] (AB270152), expandable thumbnail](https://content.abcam.com/products/images/pe-sox10-antibody-sp267-ab270152--flow-cytometry-intracellular-img175467.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-SOX10 antibody [SP267] (AB270152)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
Alternative names
Transcription factor SOX-10, SOX10
Recommended products
Rabbit Monoclonal SOX10 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
SP267
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-SOX10 antibody [SP267] (ab270152), expandable thumbnail](https://content.abcam.com/products/images/pe-sox10-antibody-sp267-ab270152--flow-cytometry-intracellular-img175467.jpg "Flow Cytometry (Intracellular) - PE Anti-SOX10 antibody [SP267] (ab270152)")
Flow Cytometry (Intracellular) - PE Anti-SOX10 antibody [SP267] (ab270152)
Flow cytometry overlay histogram showing left A375 positive cells and right negative HeLa cells stained with ab270152 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab270152) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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