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AB314270

PE Anti-STAT5a + STAT5b antibody [EPR16671-40]

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Rabbit Recombinant Monoclonal STAT5b antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

Signal transducer and activator of transcription 5B, STAT5B, STAT5, STAT5A, Signal transducer and activator of transcription 5A

1 Images
Flow Cytometry (Intracellular) - PE Anti-STAT5a + STAT5b antibody [EPR16671-40] (AB314270)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-STAT5a + STAT5b antibody [EPR16671-40] (AB314270)

Flow cytometry overlay histogram showing left K-562 positive cells and right negative PC3 stained with ab314270 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314270) (1x 106 in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in K-562 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • Carrier free

    Anti-STAT5b antibody [EPR16671-40] - BSA and Azide free (Detector)

  • Carrier free

    Anti-STAT5a + STAT5b antibody [EPR16671-40] - BSA and Azide free

  • Unconjugated

    Anti-STAT5a + STAT5b antibody [EPR16671-40]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-STAT5a + STAT5b antibody [EPR16671-40]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-STAT5a + STAT5b antibody [EPR16671-40]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16671-40

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/12500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT5a and STAT5b known jointly as members of the Signal Transducers and Activators of Transcription family play critical roles as transcription factors. They have molecular masses of approximately 94 kDa and 92 kDa respectively. These proteins share significant sequence similarity and are expressed in a wide range of tissues including hematopoietic cells liver and mammary glands. STAT5 proteins become activated in response to cytokines and growth factors where they dimerize and translocate to the cell nucleus to regulate gene expression.
Biological function summary

They are involved in mediating cytokine signaling by forming homo- or heterodimers upon phosphorylation by Janus kinases (JAKs). In the cytoplasm the formation of these dimers allows STAT5 to interact with DNA in the nucleus leading to the transcription of various genes important for cell proliferation differentiation and apoptosis. STAT5 has a significant role in cell cycle control and immune responses. The dimerization process involves complex formation and is an important part of its intracellular signaling cascade.

Pathways

STAT5a and STAT5b operate prominently within the JAK-STAT pathway. They are activated by proteins such as IL-2 IL-3 and Growth Hormone leading to transcriptional activity that affects growth and survival across various cell types. Besides the JAK-STAT pathway STAT5 interacts closely with the PI3K/AKT pathway thereby linking to cell survival signals. The cross-talk between these pathways enables fine-tuning of cellular responses to external stimuli and integrates signals from diverse receptors and ligands.

STAT5 deregulated activity associates with various cancers such as leukemia and breast cancer due to its role in promoting excessive cell proliferation and survival. Abnormal activity in STAT5 can also contribute to disorders like autoimmune diseases due to its impact on immune cell regulation. In diseases like these STAT5 interacts notably with proteins such as BCL-2 and MYC which are critical modulators of cell death and growth pathways. Understanding the involvement of STAT5 in disease mechanisms provides opportunities for developing targeted therapeutics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Carries out a dual function : signal transduction and activation of transcription (PubMed : 29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.
See full target information STAT5B

Additional targets

STAT5A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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