PE Anti-T7 tag® antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The T7 tag is a 11-amino acid peptide derived from the T7 major capsid protein commonly used as an epitope tag for protein purification and detection. This tag has a size of approximately 1371 Da (Dalton) and is highly recognizable due to its specific sequence and affinity for antibodies. Researchers frequently express the T7 tag in various systems including prokaryotic and eukaryotic cells to facilitate the purification process and Western blot analyses. It is also sometimes referred to by the notation "-7 = t/7" due to its tag characteristics.

Biological function summary

The T7 tag does not inherently perform functional roles in cellular processes; instead it serves as a tool to analyze target proteins. Researchers attach the T7 tag to proteins of interest allowing the tagged complex to be isolated using affinity tags such as biotin or alkaline phosphatase (-ALP) conjugates. The T7 tag's high affinity for its specific antibodies makes it an efficient choice for detection using Western blot methods including HRP Western blot and biotin size kDa assessment.

Pathways

The T7 tag does not directly interact with any specific biological pathways since its function as a tag is mainly technical. However it facilitates the study of pathways by enabling the isolation and analysis of proteins involved in key signaling cascades like the MAPK and PI3K/AKT pathways. Researchers use the T7 tag to explore protein-protein interactions and post-translational modifications within these pathways studying associated proteins such as kinases and regulatory enzymes.

Associated diseases and disorders

The T7 tag itself is not a contributor to pathologies but assists researchers in understanding disease mechanisms. This tag plays a critical role in studying proteins implicated in specific conditions like cancer and neurodegenerative diseases. For instance using the T7 tag scientists can study proteins altered in these diseases often in conjunction with proteins such as p53 in cancer or alpha-synuclein in neurodegenerative disorders. This research can uncover therapeutic targets and deepen understanding of disease progression.