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AB313945

PE Anti-TGN46 antibody [EPR24770-16] - Golgi Marker

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Rabbit Recombinant Monoclonal TGN46 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples.

View Alternative Names

TGN46, TGN51, TGOLN2, Trans-Golgi network integral membrane protein 2, Trans-Golgi network glycoprotein 46, Trans-Golgi network glycoprotein 48, Trans-Golgi network glycoprotein 51, Trans-Golgi network protein 2, TGN38 homolog, hTGN46, hTGN48, hTGN51

1 Images
Flow Cytometry (Intracellular) - PE Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (AB313945)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (AB313945)

Flow cytometry overlay histogram showing wild-type A549 (green line) and TGOLN2 knockout A549 stained with ab313945 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab313945) (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30 min at 22°C.

Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, TGOLN2 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TGN46 antibody [EPR24770-16] - Golgi Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TGN46 antibody [EPR24770-16] - Golgi Marker

  • Unconjugated

    Anti-TGN46 antibody [EPR24770-16] - Golgi Marker

  • Carrier free

    Anti-TGN46 antibody [EPR24770-16] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24770-16

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TGN46 also known as trans-Golgi network protein 46 is an important membrane protein associated with the trans-Golgi network. The protein has a mass of approximately 46 kDa and plays a role in the sorting and trafficking of proteins to their proper destinations within the cell. TGN46 is widely expressed in various cell types and is commonly used as a Golgi marker in cell biology studies helping to identify Golgi apparatus in cells.
Biological function summary

The protein TGN46 functions in the sorting and transport mechanisms within the cell. It does not function as a part of a large macromolecular complex but it interacts with several other proteins to facilitate the movement of proteins from the Golgi to other sites within the cell. The protein also associates with trafficking components to ensure the correct delivery of proteins to the plasma membrane or lysosomes.

Pathways

The role of TGN46 involves participation in intracellular transport and protein trafficking pathways. It interacts with clathrin-coated vesicles and works closely with proteins such as gamma-adaptin and epsin which are important for vesicle formation and transport. These interactions highlight its involvement in protein secretion and membrane transport pathways which are essential for maintaining cellular homeostasis.

TGN46 appears to have connections to neurodegenerative diseases and certain forms of cancer although the mechanisms are not fully understood. Alterations in TGN46 have been observed in Alzheimer's disease where it may intersect with proteins like amyloid precursor protein (APP) in protein misfolding and aggregation pathways. Additionally changes in TGN46 expression levels have been implicated in prostate cancer potentially due to its role in protein sorting and dysregulation of cellular trafficking processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in regulating membrane traffic to and from trans-Golgi network.
See full target information TGOLN2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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