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AB45093

PE Anti-TLR3 antibody [40C1285.6]

4

(2 Reviews)

|

(3 Publications)

Mouse Monoclonal TLR3 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TLR3 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD283, Toll-like receptor 3, TLR3

2 Images
Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (AB45093)
  • Flow Cyt (Intra)

AbReview37277****

Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (AB45093)

ab45093 staining TLR3 in Human A549 (lung epithelial) cell line by Flow Cytometry. The sample was incubated with the primary antibody (5μg/ml in 0.1% Saponin + 1% FCS in PBS) for 45 minutes at 4°C. 1 = isotype control, 2 = ab45093

This image is courtesy of an anonymous Abreview

Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (AB45093)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-TLR3 antibody [40C1285.6] (AB45093)

ab45093, at 3 ug/ 1X10^6 cells, staining Ramos cells by Intracellular flow cytometric analysis. Toll-Like-Receptor3 (open peak) and isotype negative control (shaded peak).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

40C1285.6

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human TLR3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O15455

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg/cells for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR3 also known as Toll-like receptor 3 is a protein that plays an important role in immune response mechanisms. With a molecular mass of around 104 kDa TLR3 is part of the Toll-like receptor family and expresses mostly in immune cells including dendritic cells and macrophages. It locates mainly on the endosomal membrane where it recognizes double-stranded RNA (dsRNA) which is often a sign of viral infection. TLR3 initiates signaling cascades that lead to the activation of important immune transcription factors.
Biological function summary

TLR3 acts as a sensor for viral and endogenous dsRNA. This receptor forms part of the innate immune system serving as a first line of defense against microbial infections. Upon encountering its ligand TLR3 undergoes conformational changes which allow interaction with the adaptor protein TRIF (TIR-domain-containing adapter-inducing interferon-β). This interaction triggers a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines which are essential for mounting an effective antiviral response.

Pathways

TLR3 engages in important signaling cascades like the NF-kB and IRF3 pathways. These pathways are activated by the TRIF-dependent pathway which is unique among the Toll-like receptors. TLR3 influences the expression of genes involved in the inflammatory response and apoptosis. The interactions of TLR3 with other proteins such as MyD88 and IRAK1 are indirect as TLR3 does not depend on the usual MyD88-dependent pathway. This specificity makes TLR3 a critical component in pathogen recognition and response strategies within the immune system.

TLR3 has connections with several conditions primarily viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Mutations or dysregulation of TLR3 may lead to altered immune responses contributing to the pathogenesis of these diseases. TLR3's association with proteins like TRIF and IRF3 is significant in understanding its role in these conditions. Ongoing research investigates TLR3's potential as a therapeutic target to modulate immune responses in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.
See full target information TLR3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 14:344 PubMed38031182

2023

The effect of TLR3 priming conditions on MSC immunosuppressive properties.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Tolstova,Ekaterina Dotsenko,Peter Kozhin,Svetlana Novikova,Victor Zgoda,Alexander Rusanov,Nataliya Luzgina

Frontiers in immunology 13:954984 PubMed36591257

2022

Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu M Ramamurthy,Martin Rodriguez,Hannah C Ainsworth,Jordan Shields,Diane Meares,Colin Bishop,Andrew Farland,Carl D Langefeld,Anthony Atala,Christopher B Doering,H Trent Spencer,Christopher D Porada,Graça Almeida-Porada

Clinical and vaccine immunology : CVI 18:655-60 PubMed21325490

2011

Increased cell-mediated immune responses in patients with recurrent herpes simplex virus type 2 meningitis.

Applications

Flow Cyt

Species

Unspecified reactive species

Elisabeth Franzen-Röhl,Danika Schepis,Maria Lagrelius,Kristina Franck,Petra Jones,Jan-Åke Liljeqvist,Tomas Bergström,Elisabeth Aurelius,Klas Kärre,Louise Berg,Hans Gaines
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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