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AB45097

PE Anti-TLR8 antibody [44C143]

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(6 Publications)

Mouse Monoclonal TLR8 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TLR8 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD288, UNQ249/PRO286, TLR8, Toll-like receptor 8

1 Images
Flow Cytometry (Intracellular) - PE Anti-TLR8 antibody [44C143] (AB45097)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-TLR8 antibody [44C143] (AB45097)

ab45097 at 2μg/106 cells staining Ramos cells by flow cytometry analysis. The shaded histogram represents cells without antibody, green represents isotype control and red represents ab45097.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

44C143

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human TLR8 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NR97

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2-5 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR8 also known as Toll-like receptor 8 is a member of the Toll-like receptor family and has a molecular mass of approximately 120 kDa. This receptor is mainly expressed in immune cells such as monocytes macrophages and dendritic cells. The receptor is located within endosomes and is responsible for recognizing single-stranded RNA (ssRNA) from viruses which leads to an immune response. TLR8 plays a part in detecting pathogens and activating innate immunity.
Biological function summary

TLR8 contributes to the immune system by playing a role in the innate immune response specifically in sensing viral components. TLR8 forms part of a larger receptor complex that includes MyD88 (myeloid differentiation primary response 88) an adaptor protein important for signaling processes within innate immunity. Upon recognizing ssRNA TLR8 initiates signaling cascades to trigger the release of inflammatory cytokines and other mediators that contribute to an antiviral response.

Pathways

TLR8 engages in the innate immune signaling pathway and the NF-kB pathway. These pathways are significant for the activation of immune responses and inflammation. MyD88 bridges TLR8 and downstream signaling events leading to the production and release of pro-inflammatory cytokines. In addition to MyD88 connections to molecules like IRAK4 illustrate how TLR8 signaling maintains a robust immune response to pathogen detection.

TLR8 is linked to autoimmune conditions and viral infections. The receptor's activation by viral components can contribute to chronic inflammation seen in autoimmune diseases such as systemic lupus erythematosus. Additionally aberrant TLR8 signaling is associated with increased susceptibility to certain viral infections where its relationship with MyD88 influences both protective and pathological responses in diseases. Understanding TLR8's function may unveil new therapeutic approaches to manage immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed : 25297876, PubMed : 32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed : 31778653). Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses (PubMed : 33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed : 23520111, PubMed : 25599397, PubMed : 26929371, PubMed : 33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed : 16737960, PubMed : 17932028, PubMed : 29155428).
See full target information TLR8
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:954984 PubMed36591257

2022

Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu M Ramamurthy,Martin Rodriguez,Hannah C Ainsworth,Jordan Shields,Diane Meares,Colin Bishop,Andrew Farland,Carl D Langefeld,Anthony Atala,Christopher B Doering,H Trent Spencer,Christopher D Porada,Graça Almeida-Porada

PloS one 9:e106501 PubMed25203745

2014

Asthma is associated with multiple alterations in anti-viral innate signalling pathways.

Applications

Flow Cyt

Species

Human

Antonia L Pritchard,Olivia J White,Julie G Burel,Melanie L Carroll,Simon Phipps,John W Upham

American journal of obstetrics and gynecology 210:445.e1-6 PubMed24291497

2013

Longitudinal expression of Toll-like receptors on dendritic cells in uncomplicated pregnancy and postpartum.

Applications

Unspecified application

Species

Human

Brett C Young,Aleksandar K Stanic,Britta Panda,Bo R Rueda,Alexander Panda

European journal of immunology 41:1639-51 PubMed21469104

2011

Human CD8⁺ T cells drive Th1 responses through the differentiation of TNF/iNOS-producing dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Zhen Chong,Kok Loon Wong,Gen Lin,Christopher Maolin Yang,Siew-Cheng Wong,Veronique Angeli,Paul A Macary,David Michael Kemeny

Journal of immunology (Baltimore, Md. : 1950) 182:2919-28 PubMed19234187

2009

Reversal of human papillomavirus-specific T cell immune suppression through TLR agonist treatment of Langerhans cells exposed to human papillomavirus type 16.

Applications

Flow Cyt

Species

Human

Laura M Fahey,Adam B Raff,Diane M Da Silva,W Martin Kast

Blood 108:1284-90 PubMed16638933

2006

Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ofer Levy,Eugénie E Suter,Richard L Miller,Michael R Wessels
View all publications
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Product promise

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