AB225496

PE Anti-TMEM119 antibody [106-6] - Microglial marker

RabMAb
Recombinant
What is this?

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(3 Publications)

Anti-TMEM119 antibody [106-6] - PE conjugated (ab225496) is a rabbit recombinant monoclonal antibody detecting TMEM119 in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

1 Images

Flow Cytometry (Intracellular) - PE Anti-TMEM119 antibody [106-6] - Microglial marker (AB225496)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-TMEM119 antibody [106-6] - Microglial marker (AB225496)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225496 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Phycoerythrin ab209478 (left). Cells were surface-stained with ab225496, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor^® 647 conjugated Myc-tag antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker
Unconjugated

Anti-TMEM119 antibody [106-6] - Microglial marker
Carrier free

Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

106-6

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
PE Anti-TMEM119 antibody [106-6] (ab225496) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone 106-6 is also available pre-conjugated to a variety of labels for your convenience – Anti-TMEM119 PE [106-6] (ab225496).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.

Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in bone formation and normal bone mineralization (PubMed : 20025746, PubMed : 22416756, PubMed : 26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746, PubMed : 22416756, PubMed : 22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed : 21239498, PubMed : 22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed : 24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed : 26207632).

See full target information Tmem119

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115176 PubMed39842435

2025

The microglial response to inhibition of Colony-stimulating-factor-1 receptor by PLX3397 differs by sex in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Linh H D Le,Sophia Eliseeva,Elizabeth Plunk,Kallam Kara-Pabani,Herman Li,Felix Yarovinsky,Ania K Majewska

The Journal of experimental medicine 221: PubMed38226975

2024

Aducanumab anti-amyloid immunotherapy induces sustained microglial and immune alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Mika P Cadiz,Katelin A Gibson,Kennedi T Todd,David G Nascari,Nashali Massa,Meredith T Lilley,Kimberly C Olney,Md Mamun Al-Amin,Hong Jiang,David M Holtzman,John D Fryer

PloS one 19:e0296280 PubMed38180982

2024

Distinct microglial transcriptomic signatures within the hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Sana Chintamen,Pallavi Gaur,Nicole Vo,Elizabeth M Bradshaw,Vilas Menon,Steven G Kernie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-TMEM119 antibody [106-6] - Microglial marker

Flow Cytometry (Intracellular) - PE Anti-TMEM119 antibody [106-6] - Microglial marker (AB225496)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise