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AB322526

PE Anti-TNF alpha antibody [EPR21753-109]

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Rabbit Recombinant Monoclonal TNF alpha antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images
Flow Cytometry (Intracellular) - PE Anti-TNF alpha antibody [EPR21753-109] (AB322526)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-TNF alpha antibody [EPR21753-109] (AB322526)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 3 hours then together with 300ng/ml BFA for another 3 hours (Green) / Untreated control (Magenta) cells labelling TNF alpha with ab322526 at 1/5000 dilution (0.01ug) (Magenta) and Green compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Immunocytochemistry/ Immunofluorescence - PE Anti-TNF alpha antibody [EPR21753-109] (AB322526)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-TNF alpha antibody [EPR21753-109] (AB322526)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling TNF alpha with ab322526 at 1/50 (10.0 ug/ml) dilution.

Confocal image showing cytoplasmic staining in RAW264.7 treated with LPS (100ng/ml) for 3h, together with 300ng/ml BFA for another 3h (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Carrier free

    Anti-TNF alpha antibody [EPR21753-109] - BSA and Azide free

  • Unconjugated

    Anti-TNF alpha antibody [EPR21753-109]

  • 660 APC

    APC Anti-TNF alpha antibody [EPR21753-109]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TNF alpha antibody [EPR21753-109]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TNF-alpha antibody [EPR21753-109]

  • 519 FITC

    FITC Anti-TNF alpha antibody [EPR21753-109]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21753-109

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com