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AB306108

PE Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

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Rabbit Recombinant Monoclonal Topoisomerase II alpha antibody - conjugated to PE.

View Alternative Names

TOP2, TOP2A, DNA topoisomerase 2-alpha

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 660 APC

    APC Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • HRP

    HRP Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5377

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II alpha and Topoisomerase II beta often referred to as TOP2A and TOP2B respectively are enzymes weighing approximately 170 kDa each. They are known for their role in altering the topological states of DNA during transcription and replication by creating transient double-stranded breaks in the DNA helix. TOP2A and TOP2B exhibit differential expression; TOP2A is generally more active in proliferating cells especially in the S and G2/M phases of the cell cycle while TOP2B is expressed in non-proliferating cells. The expression occurs in a variety of central tissues with notable presence in the liver lungs and brain.
Biological function summary

These enzymes are important for processes like DNA replication chromosome segregation and recombination. They don't function as part of larger complexes but perform their tasks independently. While both enzymes facilitate DNA strand passage TOP2A is more relevant during cell division ensuring that chromosomes are properly disentangled and segregated. Meanwhile TOP2B functions in regulating transcription in differentiated cells by enabling structural changes necessary for gene expression modulation.

Pathways

Topoisomerase II alpha and beta are integral to DNA replication and repair pathways. In the replication pathway they work closely with proteins like DNA polymerase to ensure DNA strands do not entangle. In the DNA damage response pathway these enzymes help resolve DNA supercoils and knots that form during replication stress. By interacting with proteins such as the breast cancer type 1 susceptibility protein (BRCA1) they contribute significantly to maintaining genome stability preventing genetic aberrations during replication.

Topoisomerase II enzymes have strong links to cancer and neurological disorders. In cancers particularly those involving rapidly dividing cells like leukemia overexpression or mutations in TOP2A can lead to unchecked cell proliferation. Interactions with proteins like p53 a tumor suppressor highlight their importance in cancer biology. In neurological disorders aberrant activity of TOP2B has connections to neurodegenerative diseases such as Alzheimer's through its role in transcription regulation. These interactions can affect proteins associated with neuronal function highlighting the enzymes' diverse impact on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed : 17567603, PubMed : 18790802, PubMed : 22013166, PubMed : 22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
See full target information TOP2A

Additional targets

DNA topoisomerase 2-beta,DNA topoisomerase 2-beta
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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