AB209927

PE Anti-TSG101 antibody [EPR7130(B)]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal TSG101 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

1 Images

Flow Cytometry (Intracellular) - PE Anti-TSG101 antibody [EPR7130(B)] (AB209927)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-TSG101 antibody [EPR7130(B)] (AB209927)

Overlay histogram showing Jurkat cells stained with ab209927 (red line). The cells were fixed with 4% formaldehyde and 80% Methanol and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209927, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

Carrier free

Anti-TSG101 [EPR7130(B)] – Goat IgG (Chimeric) – BSA and Azide Free
Carrier free

Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free
Carrier free

Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free
Unconjugated

Anti-TSG101 antibody [EPR7130(B)]
660 APC

APC Anti-TSG101 antibody [EPR7130(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-TSG101 antibody [EPR7130(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TSG101 antibody [EPR7130(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7130(B)

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.

Biological function summary

TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.

Pathways

TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.

Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed : 22315426).

See full target information Tumor susceptibility gene 101 protein

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 45:7827-7841 PubMed37886937

2023

T-Lymphocytes Activated by Dendritic Cells Loaded by Tumor-Derived Vesicles Decrease Viability of Melanoma Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Yurevich Filin,Yuriy Pavlovich Mayasin,Chulpan Bulatovna Kharisova,Anna Valerevna Gorodilova,Daria Sergeevna Chulpanova,Kristina Viktorovna Kitaeva,Albert Anatolyevich Rizvanov,Valeria Vladimirovna Solovyeva

FEBS open bio 14:740-755 PubMed37199081

2023

Endothelial cell-derived extracellular vesicles induce pro-angiogenic responses in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hüseyin Abdik,Oğuz Kaan Kırbaş,Batuhan Turhan Bozkurt,Ezgi Avşar Abdik,Taha Bartu Hayal,Fikrettin Şahin,Pakize Neslihan Taşlı

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 378:20220173 PubMed37122212

2023

Human induced pluripotent stem cell-derived cardiac myocytes and sympathetic neurons in disease modelling.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Li,Michael Edel,Kun Liu,Chris Denning,Jacob Betts,Oliver C Neely,Dan Li,David J Paterson

Current issues in molecular biology 45:571-592 PubMed36661524

2023

Cytochalasin B-Induced Membrane Vesicles from TRAIL-Overexpressing Mesenchymal Stem Cells Induce Extrinsic Pathway of Apoptosis in Breast Cancer Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Daria S Chulpanova,Tamara V Pukhalskaia,Zarema E Gilazieva,Yuliya V Filina,Milana N Mansurova,Albert A Rizvanov,Valeriya V Solovyeva

Current issues in molecular biology 44:5363-5378 PubMed36354675

2022

Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zarema Gilazieva,Daria Chulpanova,Aleksei Ponomarev,Ivan Filin,Ekaterina Garanina,Albert Rizvanov,Valeriya Solovyeva

Frontiers in immunology 13:824378 PubMed35401544

2022

Preclinical Studies on Convalescent Human Immune Plasma-Derived Exosome: Omics and Antiviral Properties to SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Neslihan Pakize Taşlı,Zeynep Burçin Gönen,Oğuz Kaan Kırbaş,Nur Seda Gökdemir,Batuhan Turhan Bozkurt,Buse Bayrakcı,Derya Sağraç,Ezgi Taşkan,Sevda Demir,Nur Ekimci Gürcan,Melike Bayındır Bilgiç,Ömer Faruk Bayrak,Hazel Yetişkin,Büşra Kaplan,Shaikh Terkıs Islam Pavel,Gökçen Dinç,Müge Serhatlı,Gamze Çakırca,Ahmet Eken,Vedat Aslan,Mehmet Yay,Musa Karakukcu,Ekrem Unal,Fethi Gül,Kemal Erdem Basaran,Yusuf Ozkul,Fikrettin Şahin,Olcay Y Jones,Şaban Tekin,Aykut Özdarendeli,Mustafa Cetin

BioNanoScience 12:293-301 PubMed35261871

2022

Analysis of the Interaction of Human Neuroblastoma Cell-Derived Cytochalasin B Induced Membrane Vesicles with Mesenchymal Stem Cells Using Imaging Flow Cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Valeriya V Solovyeva,Kristina V Kitaeva,Daria S Chulpanova,Svetlana S Arkhipova,Ivan Yu Filin,Albert A Rizvanov

Scientific reports 9:19159 PubMed31844310

2019

Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System.

Applications

Unspecified application

Species

Unspecified reactive species

Oğuz Kaan Kırbaş,Batuhan Turhan Bozkurt,Ayla Burçin Asutay,Beyza Mat,Bihter Ozdemir,Dilek Öztürkoğlu,Hülya Ölmez,Zeynep İşlek,Fikrettin Şahin,Pakize Neslihan Taşlı

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-TSG101 antibody [EPR7130(B)]

Flow Cytometry (Intracellular) - PE Anti-TSG101 antibody [EPR7130(B)] (AB209927)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise