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AB306168

PE Anti-ULK3 antibody [EPR4888]

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Rabbit Recombinant Monoclonal ULK3 antibody - conjugated to PE.

View Alternative Names

Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4888

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULK3 also known as Unc-51 Like Autophagy Activating Kinase 3 is a serine/threonine-protein kinase with a molecular mass of approximately 58 kDa. This protein plays an important role in mediating cellular processes through phosphorylation. ULK3 shows expression in various tissues including the brain and kidney. Its catalytic activity influences multiple cellular events especially those related to autophagy the cell's degradation pathway for removing damaged organelles and recycling cellular components.
Biological function summary

This protein contributes significantly through its involvement in autophagy regulation. ULK3 acts as a kinase within a multiprotein complex which includes proteins such as ATG13 and FIP200. This complex is essential for initiating autophagy by regulating the autophagosome formation. Its activity can promote cellular survival under nutrient-deprived conditions showcasing its role as an important regulatory component in cellular maintenance and stress responses.

Pathways

ULK3 takes part in critical signaling pathways such as the mTOR signaling pathway and the Hedgehog signaling pathway. In the mTOR pathway ULK3 through its kinase activity acts upstream to regulate the activity of the autophagy machinery. It shares a functional relationship with proteins like mTOR and AMPK. In the Hedgehog pathway ULK3 regulates pathway components impacting cellular growth and differentiation processes. This positions ULK3 as a modulator connecting different signaling pathways to maintain cellular homeostasis.

Researchers have reported associations with cancer and neurodegenerative diseases. Abnormal ULK3 activity can influence tumor growth and progression particularly in its regulatory role in cell proliferation and survival. Studies also suggest an involvement in neurodegenerative diseases where dysregulation in autophagy processes in which ULK3 plays a part contributes to neuronal cell death. In these contexts ULK3's interactions with proteins like mTOR and Beclin-1 may exacerbate disease progression by altering normal cellular processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand : interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH : dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy : following cellular senescence, able to induce autophagy.
See full target information Serine/threonine-protein kinase ULK3
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Product promise

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For full details, please see our Terms & Conditions

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