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AB25452

PE Anti-VCAM1 antibody [M/K-2]

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(1 Publication)

Rat Monoclonal VCAM1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1

1 Images
Flow Cytometry - PE Anti-VCAM1 antibody [M/K-2] (AB25452)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-VCAM1 antibody [M/K-2] (AB25452)

Flow cytometry analysis staining VCAM1 in mouse bone marrow cells using ab25452 at a dilution of 0.1 μg/106 cells.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M/K-2

Isotype

IgG1

Light chain type

kappa

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab25452 recognises CD106/VCAM1 (100 kDa)

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Alternative Products

Primary Antibodies

AB223982

FITC Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - FITC Anti-VCAM1 antibody [EPR5047] (AB223982)

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Primary Antibodies

AB195540

HRP Anti-VCAM1 antibody [EPR5047]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-VCAM1 antibody [EPR5047] (AB195540)

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Primary Antibodies

AB223981

PE Anti-VCAM1 antibody [EPR5047]

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Flow Cytometry (Intracellular) - PE Anti-VCAM1 antibody [EPR5047] (AB223981)

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Primary Antibodies

AB215380

Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free

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Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (AB215380)

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Primary Antibodies

AB194319

Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047]

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Primary Antibodies

AB174279

Anti-VCAM1 antibody [EPR5038(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279)

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Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

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Complementary Products

Assay Kits

AB201278

Mouse VCAM1 ELISA Kit (CD106)

Recombinant|SimpleStep

Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (AB201278)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB226

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]

Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (AB226)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Primary Antibodies

AB282575

Anti-ICAM1 antibody [EPR24639-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR24639-3] (AB282575)

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg1-kappa-monoclonal-klhg1-2-2-pe-isotype-control-ab154449'>ab154449</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
See full target information Vcam1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Leukemia research 37:1374-82 PubMed23806234

2013

Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia.

Applications

Flow Cyt

Species

Mouse

C-H Olme,R Finnon,N Brown,S Kabacik,S D Bouffler,C Badie
View all publications
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Product promise

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