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AB209446

PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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(3 Publications)

Rabbit Recombinant Monoclonal Vimentin antibody - conjugated to PE. Cytoskeleton marker. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Vimentin, VIM

3 Images
Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB209446)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB209446)

Overlay histogram showing HeLa cells stained with ab209446 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209446, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB209446)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB209446)

ab209446 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209446 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB209446)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB209446)

Overlay histogram showing HAP1 wildtype (green line) and HAP1-VIM knockout cells (red line) stained with ab209446. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209446, 0.01μg/1x106 cells OR 1/5000 dilution) for 30 min at 22°C.

A rabbit IgG isotype control antibody (ab209478) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-VIM knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • HRP

    HRP Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 660 APC

    APC Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • Unconjugated

    Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

  • Carrier free

    Anti-Vimentin antibody [EPR3776] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3776

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB52942

Anti-Vimentin (phospho S39) antibody [EP1069Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)

  • 3
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Complementary Products

Assay Kits

AB246526

Human Vimentin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB192056

Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EP1612Y]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EP1612Y] (AB192056)

  • 0
  • 0
Primary Antibodies

AB194124

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1] (AB194124)

  • 0
  • 0
Primary Antibodies

AB185048

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478'>ab209478</a> - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rhesus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
See full target information VIM
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 10:11026-11048 PubMed33042268

2020

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kok Suen Cheng,Rongbin Pan,Huaping Pan,Binglin Li,Stephene Shadrack Meena,Huan Xing,Ying Jing Ng,Kaili Qin,Xuan Liao,Benson Kiprono Kosgei,Zhipeng Wang,Ray P S Han

Communications biology 2:386 PubMed31646189

2019

Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Song Yi Ko,WonJae Lee,Hilary A Kenny,Long H Dang,Lee M Ellis,Eric Jonasch,Ernst Lengyel,Honami Naora

Methods and protocols 1: PubMed31164563

2018

Easy Employment and Crosstalk-Free Detection of Seven Fluorophores in a Widefield Fluorescence Microscope.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian C Bhakdi,Ponpan Thaicharoen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com