PE Anti-Vimentin antibody [VI-RE/1]

6 product images

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  Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

  ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

  Flow cytometry analysis (intracellular staining) of Vimentin in LEP-19 human fibroblast cell line using ab49918 anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control.

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  Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

  Vimentin Flow Cytometry (Intracellular) staining using mouse Anti-Vimentin antibody

  Separation of ESS-1 cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with ESS-1 cells stained using ab49918 (concentration in sample 0.56 μg/ml).

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  Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

  Vimentin Flow Cytometry (Intracellular) staining using mouse Anti-Vimentin antibody

  Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with ESS-1 cells stained using ab49918 (concentration in sample 0.56 μg/ml).

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  Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

  Vimentin Flow Cytometry (Intracellular) staining using mouse Anti-Vimentin antibody

  Flow cytometry analysis (intracellular staining) of Vimentin expression in LEP-19 human fibroblast cell line using PE Anti-ZNFN1A2/HELIOS antibody [22F6] ab199397. Overlay with Isotype mouse IgG1 control.

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  Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

  Separation of ESS-1 cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with ESS-1 cells stained using ab49918 at 0.56 µg/ml.