AB49918

PE Anti-Vimentin antibody [VI-RE/1]

Name: PE Anti-Vimentin antibody [VI-RE/1] (ab49918) | Abcam
Brand: Abcam Limited
SKU: AB49918
Price: 535 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal Vimentin antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.

View Alternative Names

Vimentin, VIM

6 Images

Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow cytometry analysis (intracellular staining) of Vimentin in LEP-19 human fibroblast cell line using ab49918 anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow cytometry analysis (intracellular staining) of Vimentin expression in LEP-19 human fibroblast cell line using ab199397. Overlay with Isotype mouse IgG1 control.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with ESS-1 cells stained using ab49918 (concentration in sample 0.56 μg/ml).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Separation of ESS-1 cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with ESS-1 cells stained using ab49918 (concentration in sample 0.56 μg/ml).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Unconjugated

Anti-Vimentin antibody [VI-RE/1]
660 APC

APC Anti-Vimentin antibody [VI-RE/1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

VI-RE/1

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VIM.

P08670

Specificity

The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.56-5 µg/mL", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 µg/mL", "FlowCytIntra-species-notes": "" }, "Pig": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 µg/mL", "FlowCytIntra-species-notes": "" } } }

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Product details

Conjugated with R-Phycoerythrin (PE) under optimum conditions.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibodies – protein A (G) affinity chromatography before conjugation. Conjugated antibodies –site exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23906 PubMed40615435

2025

Real-time assessment of circulating tumor cells refines the indication for HER2-targeted therapy in metastatic gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuaki Kimura,Koichi Suzuki,Sawako Tamaki,Iku Abe,Yuhei Endo,Kosuke Ichida,Yuta Muto,Fumiaki Watanabe,Masaaki Saito,Kazuo Takeda,Toshiki Rikiyama

Anticancer research 42:407-417 PubMed34969751

2022

High-purity Isolation for Genotyping Rare Cancer Cells from Blood Using a Microfluidic Chip Cell Sorter.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Ikeda,Yasuhiro Koh,Jun Oyanagi,Shunsuke Teraoka,Masayuki Ishige,Yuu Fujimura,Kazuo Takeda,Nahomi Tokudome,Yuichi Ozawa,Hiroki Ueda,Nobuyuki Yamamoto

Oncotarget 9:7522-7533 PubMed29484129

2018

Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Xiu Guo,Kuang Hong Neoh,Xiao-Hong Chang,Yukun Sun,Hong-Yan Cheng,Xue Ye,Rui-Qiong Ma,Ray P S Han,Heng Cui

PloS one 6:e21221 PubMed21712999

2011

Reconstruction of endometrium from human endometrial side population cell lines.

Applications

Flow Cyt

Species

Human

Irene Cervelló,Aymara Mas,Claudia Gil-Sanchis,Laura Peris,Amparo Faus,Philippa T K Saunders,Hilary O D Critchley,Carlos Simón

Cell cycle (Georgetown, Tex.) 10:2008-16 PubMed21558812

2011

Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Devraj Basu,Kathleen T Montone,Li-Ping Wang,Phyllis A Gimotty,Rachel Hammond,J Alan Diehl,Anil K Rustgi,John T Lee,Kati Rasanen,Gregory S Weinstein,Meenhard Herlyn

Oncogene 29:4170-82 PubMed20498638

2010

Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity.

Applications

Flow Cyt

Species

Unspecified reactive species

D Basu,T-T K Nguyen,K T Montone,G Zhang,L-P Wang,J A Diehl,A K Rustgi,J T Lee,G S Weinstein,M Herlyn

Breast cancer research : BCR 11:R82 PubMed19906290

2009

Dynamic regulation of CD24 and the invasive, CD44posCD24neg phenotype in breast cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Meyer,Jodie M Fleming,Mustapha A Ali,Mitchell W Pesesky,Erika Ginsburg,Barbara K Vonderhaar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Vimentin antibody [VI-RE/1]

Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (AB49918)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (7)

Product promise