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AB320172

PE Anti-WDR77 antibody [EPR10709(2)] - C-terminal

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Rabbit Recombinant Monoclonal WDR77 antibody - conjugated to PE.

View Alternative Names

MEP50, WD45, HKMT1069, Nbla10071, WDR77, Methylosome protein WDR77, Androgen receptor cofactor p44, Methylosome protein 50, WD repeat-containing protein 77, p44/Mep50, MEP-50

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • 660 APC

    APC Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • Unconjugated

    Anti-WDR77 antibody [EPR10709(2)] - C-terminal

  • Carrier free

    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10709(2)

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR77 also known as MEP50 is a protein with a mass of about 37 kDa. It functions as a cofactor involved in methylation processes often forming part of protein complexes with PRMT5. WDR77 locates within the cell nucleus and is widely expressed in human tissues with higher levels observed in reproductive organs like the testis and prostate. The protein interacts with other proteins to affect transcription and RNA processing.
Biological function summary

WDR77 interacts with the protein arginine methyltransferase 5 (PRMT5) and contributes to the assembly and stabilization of methyltransferase complexes. This role is essential for its participation in the methylation of arginine residues on histones and other proteins which regulates gene expression. WDR77 influences cell proliferation and differentiation by modulating these processes especially in germ cells and prostate epithelial cells.

Pathways

WDR77 is an important player in the transcriptional repression pathway affecting histone modification. It connects intimately with important pathways like the PI3K/AKT signaling and cell cycle regulation. Through its interaction with PRMT5 WDR77 takes part in regulatory networks impacting gene silencing chromatin remodeling and the overall gene expression mechanism. The involvement of WDR77 in these pathways emphasizes its relationship with PRMT5 and other methylation-associated proteins.

WDR77 has links to prostate cancer and lung cancer. These associations may result from its role in cell cycle control and proliferation which becomes dysregulated in oncogenesis. WDR77's interaction with PRMT5 is critical in such conditions as changes in their activity can alter normal cell growth patterns. Research indicates that abnormal expression or function of WDR77 can lead to increased tumorigenesis in specific tissues emphasizing the protein's potential as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed : 11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed : 23071334).
See full target information WDR77
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