Armenian hamster Monoclonal ZNFN1A2/HELIOS antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IKZF2 aa 50-150.
IgG
Armenian hamster
PE
Ex: 480;565nm, Em: 578nm
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS, 0.2% BSA
Liquid
Monoclonal
Flow Cyt (Intra) | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 10 µL for 106 Cells | Notes Use at 10 μl / 100 μl of whole blood |
Select an associated product type
Associates with Ikaros at centromeric heterochromatin.
HELIOS, ZNFN1A2, IKZF2, HELIOS, ZNFN1A2, Zinc finger protein Helios, Ikaros family zinc finger protein 2
Armenian hamster Monoclonal ZNFN1A2/HELIOS antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IKZF2 aa 50-150.
IgG
Armenian hamster
PE
Ex: 480;565nm, Em: 578nm
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS, 0.2% BSA
Liquid
Monoclonal
22F6
Size-exclusion chromatography
The antibody is purified from cell culture supernatant by protein-A affinity chromatography (Purity: > 95% (by SDS-PAGE)) and then conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Blue Ice
+4°C
+4°C
Store in the dark
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This supplementary information is collated from multiple sources and compiled automatically.
ZNFN1A2 also known as HELIOS is a transcription factor with an estimated mass of approximately 65 to 70 kDa. It is a member of the Ikaros family of zinc finger proteins which are essential in regulating gene expression. HELIOS is expressed mainly in the thymus with significant levels also found in peripheral T cells. This protein plays a role in lymphocyte development and differentiation influencing the function and survival of T cells.
ZNFN1A2 performs critical functions in maintaining T cell homeostasis and promoting regulatory T cell development. It forms part of transcriptional complexes that regulate gene expression necessary for immune response modulation. ZNFN1A2 interacts with other members of the Ikaros family sharing overlapping roles in immune cell regulation which highlights its importance in immune regulation.
ZNFN1A2 integrates into critical signaling networks related to T cell regulation particularly the T cell receptor (TCR) signaling pathway. Within these pathways HELIOS collaborates with proteins such as FOXP3 and GATA3 to mediate immune tolerance and maintain immune system balance. These interactions underline the protein's involvement in modulating immune responses and preventing autoimmunity.
ZNFN1A2 demonstrates significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where dysregulation leads to improper T cell function. Furthermore it is implicated in leukemias particularly T-cell acute lymphoblastic leukemia (T-ALL) due to altered expression affecting T cell maturation. In these diseases ZNFN1A2 often interacts with proteins like AIRE and BCL11B influencing disease progression and clinical outcomes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Intracellular flow cytometric analysis of human peripheral blood lymphocytes labeling ZNFN1A2/HELIOS with ab199397 (10μL ab199397 / 100μL at4°C, in the dark). The cells were first labeled withanti-CD4 Pacific BlueTM, fixed and permeabilized .
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