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Armenian hamster Monoclonal ZNFN1A2/HELIOS antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IKZF2 aa 50-150.

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Images

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397), expandable thumbnail

Key facts

Isotype

IgG

Host species

Armenian hamster

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS, 0.2% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human IKZF2 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKS7

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested

Tested
Tested

Species

Human

Dilution info

10 µL for 106 Cells

Notes

Use at 10 μl / 100 μl of whole blood

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Associates with Ikaros at centromeric heterochromatin.

Alternative names

Recommended products

Armenian hamster Monoclonal ZNFN1A2/HELIOS antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IKZF2 aa 50-150.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human IKZF2 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKS7
Clone number

22F6

Purification technique

Size-exclusion chromatography

Concentration
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Purification notes

The antibody is purified from cell culture supernatant by protein-A affinity chromatography (Purity: > 95% (by SDS-PAGE)) and then conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZNFN1A2 also known as HELIOS is a transcription factor with an estimated mass of approximately 65 to 70 kDa. It is a member of the Ikaros family of zinc finger proteins which are essential in regulating gene expression. HELIOS is expressed mainly in the thymus with significant levels also found in peripheral T cells. This protein plays a role in lymphocyte development and differentiation influencing the function and survival of T cells.

Biological function summary

ZNFN1A2 performs critical functions in maintaining T cell homeostasis and promoting regulatory T cell development. It forms part of transcriptional complexes that regulate gene expression necessary for immune response modulation. ZNFN1A2 interacts with other members of the Ikaros family sharing overlapping roles in immune cell regulation which highlights its importance in immune regulation.

Pathways

ZNFN1A2 integrates into critical signaling networks related to T cell regulation particularly the T cell receptor (TCR) signaling pathway. Within these pathways HELIOS collaborates with proteins such as FOXP3 and GATA3 to mediate immune tolerance and maintain immune system balance. These interactions underline the protein's involvement in modulating immune responses and preventing autoimmunity.

Associated diseases and disorders

ZNFN1A2 demonstrates significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where dysregulation leads to improper T cell function. Furthermore it is implicated in leukemias particularly T-cell acute lymphoblastic leukemia (T-ALL) due to altered expression affecting T cell maturation. In these diseases ZNFN1A2 often interacts with proteins like AIRE and BCL11B influencing disease progression and clinical outcomes.

Product promise

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1 product image

  • Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (ab199397), expandable thumbnail

    Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (ab199397)

    Intracellular flow cytometric analysis of human peripheral blood lymphocytes labeling ZNFN1A2/HELIOS with ab199397 (10μL ab199397 / 100μL at4°C, in the dark). The cells were first labeled withanti-CD4 Pacific BlueTM, fixed and permeabilized .

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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