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AB199397

PE Anti-ZNFN1A2/HELIOS antibody [22F6]

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(1 Publication)

Armenian hamster Monoclonal ZNFN1A2/HELIOS antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IKZF2 aa 50-150.

View Alternative Names

HELIOS, ZNFN1A2, IKZF2, Zinc finger protein Helios, Ikaros family zinc finger protein 2

4 Images
Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)

Intracellular flow cytometric analysis of human peripheral blood lymphocytes labeling ZNFN1A2/HELIOS with ab199397 (10μL ab199397 / 100μL at4°C, in the dark). The cells were first labeled withanti-CD4 Pacific BlueTM, fixed and permeabilized .

Flow Cytometry - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD4 (MEM-241) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and intracellular staining of human lymphocytes using ab199397 (10 ul reagent / 100 ul of peripheral whole blood).

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using ab199397 (10 μl reagent/100 μl of peripheral whole blood).

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Anti-ZNFN1A2/HELIOS antibody [22F6] (AB199397)

Separation of human CD4 positive Helios positive T cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using ab199397 (10 ul reagent / 100 ul of peripheral whole blood).

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

22F6

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human IKZF2 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UKS7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p>Use at 10 μl / 100 μl of whole blood</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The antibody is purified from cell culture supernatant by protein-A affinity chromatography (Purity: > 95% (by SDS-PAGE)) and then conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNFN1A2 also known as HELIOS is a transcription factor with an estimated mass of approximately 65 to 70 kDa. It is a member of the Ikaros family of zinc finger proteins which are essential in regulating gene expression. HELIOS is expressed mainly in the thymus with significant levels also found in peripheral T cells. This protein plays a role in lymphocyte development and differentiation influencing the function and survival of T cells.
Biological function summary

ZNFN1A2 performs critical functions in maintaining T cell homeostasis and promoting regulatory T cell development. It forms part of transcriptional complexes that regulate gene expression necessary for immune response modulation. ZNFN1A2 interacts with other members of the Ikaros family sharing overlapping roles in immune cell regulation which highlights its importance in immune regulation.

Pathways

ZNFN1A2 integrates into critical signaling networks related to T cell regulation particularly the T cell receptor (TCR) signaling pathway. Within these pathways HELIOS collaborates with proteins such as FOXP3 and GATA3 to mediate immune tolerance and maintain immune system balance. These interactions underline the protein's involvement in modulating immune responses and preventing autoimmunity.

ZNFN1A2 demonstrates significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where dysregulation leads to improper T cell function. Furthermore it is implicated in leukemias particularly T-cell acute lymphoblastic leukemia (T-ALL) due to altered expression affecting T cell maturation. In these diseases ZNFN1A2 often interacts with proteins like AIRE and BCL11B influencing disease progression and clinical outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor, which stabilizes the noninflammatory phenotype of regulatory T cells (Tregs) (By similarity). May possibly act via STAT5-mediated signaling (By similarity). Binds IL2 promoter and represses its transcription, possibly by epigenetic silencing, inducing deacetylation of core histones in the IL2 promoter (PubMed : 37316189). In Tregs, required for FOXP3 recruitment to the IL2 promoter (By similarity). Required for outer hair cells maturation and, consequently, for hearing (By similarity).
See full target information IKZF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 43:710-717 PubMed37313811

2023

[RITA selectively inhibits proliferation of -deficient cutaneous melanoma cells ].

Applications

Unspecified application

Species

Unspecified reactive species

W Shi,X Liu,G Zhang,L Ye,R Zhou,Y Li,L Yu
View all publications
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