AB277626

Anti-PEA15 antibody [4D2]

Name: Anti-PEA15 antibody [4D2] (ab277626) | Abcam
Brand: Abcam Limited
SKU: AB277626
Price: 515 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal PEA15 antibody. Suitable for IHC, WB and reacts with Rat, Mouse, Cow samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PEA15.

View Alternative Names

Astrocytic phosphoprotein PEA-15, 15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enriched in diabetes, PED, PEA15

2 Images

Supplier Data

Immunohistochemistry - Anti-PEA15 antibody [4D2] (AB277626)

Perfusion-fixed, free floating sections of rat brain section stained for PEA15 using ab277626 (red) at 1/1000 dilution and counterstained with rabbit pAb to GFAP (green) at 1/5000 dilution in immunohistochemical analysis. The blue Hoechst is staining nuclear DNA.

Supplier Data

Western blot - Anti-PEA15 antibody [4D2] (AB277626)

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Western blot - Anti-PEA15 antibody [4D2] (ab277626)

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Western blot - Anti-PEA15 antibody [4D2] (ab277626) at 1/1000 dilution

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Molecular weight ladder

Lane 2:

rat whole brain

Lane 3:

rat cerebellum

Lane 4:

mouse whole brain

Lane 5:

cow cortex

Lane 6:

cow cerebellum

Predicted band size: 15 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Cow

Applications

WB, IHC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PEA15.

Q15121

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Rat": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "1/1000 - 1/2000", "IHC-species-notes": "transcardial perfusion-fixed with 4% paraformaldehyde for 24 hours, free floating sections", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Cow": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from Tissue Culture supernatant.

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEA15 also known as phosphoprotein enriched in astrocytes 15 or PED is a multifunctional protein weighing around 15 kDa. Mechanically PEA15 acts as a death effector domain (DED)-containing protein that modulates diverse cellular processes by binding to and inhibiting other DED-containing proteins. This protein shows wide expression notably in the central nervous system including the brain where it exerts critical roles in signaling networks.

Biological function summary

PEA15 plays pivotal roles in promoting cellular survival and regulating apoptosis within various cell types. It operates by modulating pathways like apoptosis and cell proliferation often forming complexes with other DED-containing proteins such as FADD and caspase-8. By interacting with these proteins PEA15 can maintain cellular stability and manage stress responses illustrating its significant contribution to cellular homeostasis.

Pathways

PEA15 is integral in mediating the extracellular signal-regulated kinase (ERK) and MAP kinase pathways. As PEA15 binds to ERK1/2 it retains these kinases in the cytoplasm impeding their translocation to the nucleus and therefore affecting the transcription of target genes. Furthermore its interactions with Ras-related proteins indicate its involvement in the regulation of cell growth and differentiation showing its broad influence on cellular signaling cascades.

PEA15 correlates with neurological conditions including Alzheimer's disease and gliomas. In Alzheimer's disease PEA15 modulates neuroinflammation and amyloid precursor protein processing collaborating with proteins such as tau and amyloid-beta. In gliomas alterations in PEA15 expression influence tumor progression and treatment susceptibility highlighting important intersections with oncogenic factors like EGFR and PTEN. These associations spotlight PEA15 as a potential therapeutic target in both neurodegenerative and cancerous disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

See full target information PEA15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e23750 PubMed38192850

2024

Formononetin ameliorates cisplatin-induced hair cell death via activation of the PI3K/AKT-Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Li,Jingfang Wu,Huiqian Yu,Xiaoling Lu,Yusu Ni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PEA15 antibody [4D2]

Immunohistochemistry - Anti-PEA15 antibody [4D2] (AB277626)

Western blot - Anti-PEA15 antibody [4D2] (AB277626)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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