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AB70425

Anti-Pea3 antibody [1A2G3]

3

(2 Reviews)

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(8 Publications)

Mouse Monoclonal Pea3 antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETV4 aa 50-150.

View Alternative Names

E1AF, PEA3, ETV4, ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3

3 Images
Flow Cytometry - Anti-Pea3 antibody [1A2G3] (AB70425)
  • Flow Cyt

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Flow Cytometry - Anti-Pea3 antibody [1A2G3] (AB70425)

Overlay histogram showing K562 cells stained with ab70425 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70425, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Western blot - Anti-Pea3 antibody [1A2G3] (AB70425)
  • WB

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Western blot - Anti-Pea3 antibody [1A2G3] (AB70425)

All lanes:

Western blot - Anti-Pea3 antibody [1A2G3] (ab70425) at 1/500 dilution

Lane 1:

Truncated Trx-Pea3 recombinant protein

Lane 2:

Full length Pea3 hIgGFc transfected CHO-K1 cell lysate

Predicted band size: 54 kDa

Observed band size: 24 kDa,60 kDa

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Western blot - Anti-Pea3 antibody [1A2G3] (AB70425)
  • WB

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Western blot - Anti-Pea3 antibody [1A2G3] (AB70425)

All lanes:

Western blot - Anti-Pea3 antibody [1A2G3] (ab70425) at 1/2000 dilution

All lanes:

Cell lysates prepared from K562 cells at 100 µg

Secondary

All lanes:

HRP-conjugated Goat polyclonal to mouse IgG1

Predicted band size: 54 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A2G3

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ETV4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P43268

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pea3 also called PEA3 or ETV4 functions as a transcription factor from the ETS family. It has a mass of approximately 55 kDa. This protein regulates gene expression by binding to specific DNA sequences. Pea3 is mainly expressed in the brain lung and liver tissues where it influences the transcription of a range of target genes. It plays an important role in mediating cellular responses to extracellular signals.
Biological function summary

Pea3 influences key processes like cell proliferation differentiation and migration. It often operates within larger protein complexes to execute these roles. Pea3 has been shown to regulate genes involved in development and oncogenic processes by interacting with co-factors in transcriptional complexes. By activating or repressing the transcription of specific genes Pea3 contributes to the dynamic regulation of cellular environments.

Pathways

Several intracellular signaling pathways involve Pea3 in integrating cellular responses to external stimuli. Notably Pea3 plays a role in the MAPK/ERK pathway where it works alongside proteins like ERK and other transcription factors to mediate cell growth and differentiation. Pea3 also participates in the PI3K/AKT pathway influencing cell survival and metabolism. In these pathways Pea3 acts as a bridge linking extracellular signals to transcriptional outputs.

Pea3 connects to cancer progression and metastasis. Overexpression of Pea3 is associated with various cancers including breast and lung cancer where it interacts with proteins like matrix metalloproteinases (MMPs) to promote metastasis. Pea3 also links to neurological disorders by influencing genes involved in brain development and function demonstrating its widespread significance in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator (PubMed : 19307308, PubMed : 31552090). May play a role in keratinocyte differentiation (PubMed : 31552090).. (Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'.
See full target information ETV4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 30: PubMed39370816

2024

ETV4‑mediated transcriptional activation of SLC12A5 exacerbates ferroptosis resistance and glucose metabolism reprogramming in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Yanyan Dai,Fengxiang Wang

Experimental physiology 106:2148 PubMed33876502

2021

Retracted: Transcription activation of microRNA-25 by PEA3 augments progression of gastric cancer through suppressing SIK1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yu,Qingchun Li,Tong Chen,Huaiyu Zhang,Xiaofeng Cui,Kexin Shen

Molecular therapy. Methods & clinical development 21:492-506 PubMed33997099

2021

Discovery of key genes as novel biomarkers specifically associated with HPV-negative cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Liu,Yichi Xu,Wenxiao Jiang,Huihui Ji,Zhi-Wei Wang,Xueqiong Zhu

Cancer cell international 21:17 PubMed33407520

2021

Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Leng,Zhixian Chen,Hui Ding,Xiaoxu Zhao,Li Qin,Yunlong Pan

Journal of Cancer 11:6556-6564 PubMed33046976

2020

Icaritin reduces prostate cancer progression via inhibiting high-fat diet-induced serum adipokine in TRAMP mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Wu,Xingbo Long,Chen Yang,Huan Chen,Christina Sharkey,Khalid Rashid,Mengbo Hu,Yufei Liu,Qi Huang,Qi Chen,Jimeng Hu,Haowen Jiang

Cancer cell international 18:25 PubMed29467595

2018

Increased ETV4 expression correlates with estrogen-enhanced proliferation and invasiveness of cholangiocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ekapot Singsuksawat,Chanitra Thuwajit,Komgrid Charngkaew,Peti Thuwajit

Molecular cancer research : MCR 10:1607-19 PubMed22833572

2012

Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity.

Applications

WB

Species

Human

Stephan Gysin,Jesse Paquette,Martin McMahon

Disease models & mechanisms 4:548-55 PubMed21504907

2011

K-Ras and β-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladder.

Applications

IHC

Species

Unspecified reactive species

Imran Ahmad,Lukram Babloo Singh,Mona Foth,Carol-Ann Morris,Makoto Mark Taketo,Xue-Ru Wu,Hing Y Leung,Owen J Sansom,Tomoko Iwata
View all publications
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